Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to protect 1 μg λ DNA in 1 hour at 65°C in a total reaction volume of 20 μl against cleavage by TaqI restriction endonuclease. Reaction Conditions
1X rCutSmart™ Buffer
Supplement with 640 µM S-adenosylmethionine (SAM)
Incubate at 65°C
1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)
Control Protection Assay
One unit of TaqI Methyltransferase is incubated with 1 µg of λ DNA in 20 µl 1X rCutSmart Buffer, supplemented with 640 µM S-adenosylmethionine, for one hour at 65°C. The extent of protection by TaqI Methyltransferase is determined by the addition of 10 units of TaqI-v2 restriction endonuclease in 30 µl 1X rCutSmart Buffer followed by incubation at 65°C for 15 minutes. Results are analyzed on agarose gel. Storage Buffer
10 mM Tris-HCl
100 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
No Molecular Weight
Theoretical: 47845 daltons