Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C. Reaction Conditions
1X NEBuffer™ 2
Incubate at 37°C
1X NEBuffer™ 2
50 mM NaCl
10 mM Tris-HCl
10 mM MgCl2
1 mM DTT
(pH 7.9 @ 25°C)
Storage Buffer
25 mM Tris-HCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
75°C for 20 minutes Molecular Weight
Theoretical: 103000 daltons
5' - 3' Exonuclease
Yes 3' - 5' Exonuclease
Yes Strand Displacement
No Unit Assay Conditions
1X NEBuffer 2, 33 μM dNTPs including [3H]-dTTP and 70 μg/ml denatured herring sperm DNA. Error Rate
< 9x10-6bases