Product Class: Other

9°N™ Reverse Gyrase
Gray property icon for NEBU Gray property icon for cloned at NEB recombinant Gray property icon for incubation temperature 80° Gray property icon for heat inactivation no

Product Introduction

Illustration of Reverse Gyrase relaxing and then supercoiling DNA

 

  • Thermostable Type IA Topoisomerase
  • Positively supercoils double-stranded closed-circular DNA
  • This is an Enzyme for Innovation (EFI). EFI is a project initiated by NEB to provide unique enzymes to the scientific community in the hopes of enabling the discovery of new and innovative applications. These enzymes have interesting properties and unique specificities.

 

Bioz Badge Exists : True
Catalog # Size Concentration
M0200S 250 units 5000 units/ml

Product Information

Description

9°N™ Reverse Gyrase is a thermostable Type IA topoisomerase that introduces positive supercoils into double-stranded closed-circular DNA in the presence of ATP (1).

Product Source

An E. coli strain that carries a cloned gene from a thermophilic marine archaea Thermococcus sp. (strain 9°N) (2) with a C-terminal His-tag.
This product is related to the following categories:
DNA Modifying Enzymes & Cloning Technologies,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • NEB # Stored at (°C)
    M0200S -20
    Component Name Component # Stored at (°C) Amount Concentration
    9°N™ Reverse Gyrase M0200SVIAL -20 1 x 0.05 ml 5,000 units/ml
    Gyrase Reaction Buffer B7636SVIAL -20 1 x 1 ml 5 X

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to supercoil 0.5 µg of relaxed pUC19 plasmid in 15 minutes at 80°C, in a total reaction volume of 40 µl.

Reaction Conditions

1X Gyrase Reaction Buffer
Incubate at 80°C

Heat Inactivation

No

Features

  • Introduces positive supercoiling in the presence of ATP
  • Enhanced reaction buffer for improved activity
  • Thermostable for high temperature workflow applications

Product Notes

  1. The reaction requires ATP and Mg2+ (3, 4). The supplied Gyrase Reaction Buffer incorporates these essential components within an optimized formulation, thereby facilitating enhanced gyrase activity.
  2. In the absence of ATP, the enzyme will nick circular DNA (5).

References

  1. Lulchev, P., and Klostermeier, D. (2014). NAR. 42 (13), 8200-8213.
  2. Thermococcus sp. (strain 9°N-7) isolated by Dr. Holger Jannasch (1991). Woods Hole Oceanographic Institute.
  3. Hsieh, TS., and Capp, C. (2005). 208 (21), 20467-20475. JBC.
  4. Lee, H. et al. (2025). 53 (12). PubMedID: gkaf581 NAR.
  5. Jamroze, A. et al. (2013). 289 (6), 3231-3243. JBC.

FAQs & Troubleshooting

FAQs

  1. Can DNA Gyrase (E. coli) (NEB #M7636) and 9°N™ Reverse Gyrase (NEB #M0200) act on linear DNA?
  2. Can DNA Gyrase (E. coli) (NEB #M7636) and 9°N™ Reverse Gyrase (NEB #M0200) create supercoils in both closed and open circular DNA?
  3. Are DNA Gyrase (E. coli) (NEB #M7636) and 9°N™ Reverse Gyrase (NEB #M0200) compatible with transformation or transfection workflows?
  4. Are there contaminants that inhibit DNA Gyrase (E. coli) (NEB #M7636) and 9°N™ Reverse Gyrase (NEB #M0200)?
  5. How should DNA treated with DNA Gyrase (E. coli) (NEB #M7636), Topoisomerase I (NEB #M0301), or 9°N™ Reverse Gyrase (NEB #M0200) be run on a gel?
  6. Is the linking number (ΔLk) or the superhelical density (σ) that results from the DNA Gyrase (E.coli) (NEB #M7636) or 9°N™ Reverse Gyrase (NEB #M0200) reaction fixed or random?
  7. Will 9°N™ Reverse Gyrase (NEB #M0200) add additional positive supercoils in DNA that is already partially supercoiled?
  8. Does 9°N™ Reverse Gyrase (NEB #M0200) work in other NEBuffers?
  9. Does 9°N™ Reverse Gyrase (NEB #M0200) demonstrate additional activities?
  10. What is the reaction temperature range for 9°N™ Reverse Gyrase (NEB #M0200)?
  11. Does nicking activity occur at other temperatures for 9°N™ Reverse Gyrase (NEB #M0200)?
  12. What is the difference between DNA Gyrase (E. coli) (NEB #M7636), Topoisomerase I (E. coli) (NEB #M0301), and 9°N™ Reverse Gyrase (NEB #M0200)?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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