Product Class: Kit

NEBNext® Library Quant DNA Standards

The qPCR standards included in this product are intended for use with libraries prepped for sequencing on Illumina® instruments.

 

Product Introduction

NEBNext Library Quant DNA Standards are a set of six standards, defining a curve from 100 pM to 0.001 pM, intended for use with the NEBNext Library Quant Master Mix included in the NEBNext Library Quant Kit for Illumina (NEB #E7630).

  • Additional volume of the same standards included in the NEBNext Library Quant Kit for Illumina
  • qPCR-based library quant based on either four or six standards
 
Bioz Badge Exists : True
Catalog # Size Concentration
E7642S 500 reactions

Product Information

Description

For use with the NEBNext Library Quant Master Mix included in the NEBNext Libarary Quant Kit for Illumina, this product includes six tubes of standards (100 pM to 0.001 pM) providing extra volume for users who require it. Protocols included in the (NEB #E7630) product manual detail the process for setting up standard curves using either four or six standards, building flexibility into the workflow.

The NEBNext Library Quant Kit for Illumina offers a streamlined protocol and reduced lot-to-lot variability as compared to similar library quantification kits.
 

Figure 1: Typical amplification and standard curves for the NEBNext Library Quant Kit

Typical results from the NEBNext Library Quant Kit on a Bio-Rad CFX96 Touch (A) and an Applied Biosystems 7500 Fast real-time qPCR instrument (B). Amplification curves are shown on the left and resulting standard curves on the right. All default settings were used on both platforms. ABI 7500 Fast assay included 1X ROX (Low Concentration) and the ROX normalization.


This product is related to the following categories:
NGS Library Quantitation,

Kit Components

Kit Components

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • NEB # Stored at (°C)
    E7642S -20
    Component Name Component # Stored at (°C) Amount Concentration
    NEBNext® Library Quant DNA Standard 1 E6886AAVIAL -20 1 x 0.12 ml 100 pM
    NEBNext® Library Quant DNA Standard 2 E6887AAVIAL -20 1 x 0.12 ml 10 pM
    NEBNext® Library Quant DNA Standard 3 E6888AAVIAL -20 1 x 0.12 ml 1 pM
    NEBNext® Library Quant DNA Standard 4 E6889AAVIAL -20 1 x 0.12 ml 0.1 pM
    NEBNext® Library Quant DNA Standard 5 E6890AAVIAL -20 1 x 0.12 ml 0.01 pM
    NEBNext® Library Quant DNA Standard 6 E6891AAVIAL -20 1 x 0.12 ml 0.001 pM

Properties & Usage

Application Features

  • Additional volume of the same standards included in the NEBNext Library Quant Kit for Illumina 
  • qPCR-based library quant based on either four or six standards
 

FAQs & Troubleshooting

FAQs

  1. How are the new 6 standards different from the old 4 standards?
  2. What are the advantages of the qPCR-based NEBNext Library Quant kits compared to other methods of quantitating NGS libraries?
  3. What libraries can be quantitated with the NEBNext® Library Quant Kit for Illumina®?
  4. How much should I dilute my library to run in the Quant Kit?
  5. Can I run the NEBNext Library Quant Kit on my qPCR machine?
  6. Which ROX should I use for my qPCR machine?
  7. Are 4 standards enough for a qPCR quantitation?
  8. Does the library quant require a size correction?
  9. What other materials/information do I need to run an NEBNext® Library Quant Kit?
  10. How many libraries can I quantitate at a time?
  11. How long is a run of the Quant Kit?
  12. Do you provide any analysis tools to help with the quantitation?
  13. Can I see adaptor dimers as a measure of library quality?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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