This product has been discontinued as of June 16, 2025. As an alternative to the EM-seq™ Primer Pairs, users can purchase the NEBNext LV Unique Dual Index Primer Pairs (Sets 1-5) (NEB #E3400, E3402, E3404, E3406, E3408, E3390, E3392). The EM-seq Adaptor will be available in the NEBNext Enzymatic Methyl-seq Kit ( NEB #E7120). This change does not impact users of NEBNext Enzymatic Methyl-seq v2 Kit and/or Conversion Module.
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Catalog # E7140 was discontinued on June 16, 2025
Product Introduction
NEBNext Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs) is compatible with Illumina® sequencing and includes the NEBNext EM-seq™ Adaptor, and NEBNext PCR primers that enable incorporation of unique index pairs during library amplification. The index primers are provided as pre-mixed unique pairs of i5 and i7 index primers, packaged in a single-use 96-well plate with a pierceable foil seal, or in individual vials.
Although optimized for the EM-seq workflow, the NEBNext EM-seq Adaptor is also compatible with bisulfite sequencing workflows.
- Optimized for the EM-seq workflow
- Compatible with bisulfite sequencing workflows
- Addresses the “barcode hopping” issue seen with some Illumina sequencing instruments
- Increases sample identification specificity with unique dual index primer pairs
Storage temperature: -20°C.
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