Description
The SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit utilizes Loop-Mediated Isothermal Amplification (LAMP) to detect SARS-CoV-2 nucleic acid. The kit is available for research use only and includes WarmStart Colorimetric LAMP 2X Master Mix with UDG and a primer mix targeting the N and E regions of the viral genome. Controls are provided to verify assay performance, and include an internal control primer set and a positive control template. Guanidine hydrochloride has been found to increase the speed and sensitivity of the RT-LAMP reaction and is also included.
WarmStart Colorimetric LAMP 2X Master Mix with UDG is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of LAMP and RT-LAMP reactions. The inclusion of thermolabile UDG and dUTP in the master mix reduces the possibility of carryover contamination between reactions.
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This system provides a fast, clear visual detection of amplification based on the production of protons and the subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction. The decrease in pH produces a change in solution color from pink to yellow. The total protocol time is 30 minutes and only requires a simple heating device that can reach 65°C.
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A positive detection of the SARS-CoV-2 RNA sequence would be indicated by a yellow color (amplification occurred, protons released, pH-dependent color change from pink to yellow), while a negative result would be indicated by a pink color (no amplification occurred, no protons released, no color change).
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LAMP has been used in novel workflows by scientists around the world to further COVID-19 research. To access application notes, selected preprints, and to learn more about NEB’s COVID-19 Researcher Spotlight series, click here.
- This product is related to the following categories:
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Isothermal Amplification & Strand Displacement Products,
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PCR, qPCR & Amplification Technologies Products,
- This product can be used in the following applications:
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Loop-Mediated Isothermal Amplification,
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Isothermal Amplification,
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DNA Amplification, PCR & qPCR
Properties & Usage
Materials Required but not Supplied
Disposable powder-free gloves and any additional PPE required
P2/P10, P200, and P1000 aerosol barrier tips
Sterile, nuclease-free 1.5 ml microcentrifuge tubes
Sterile, nuclease-free 2.0 ml, 5.0 ml, or 15 ml tubes
0.2 ml PCR reaction tube strips with separated tubes and lids (e.g., VWR 20170-004) or attached caps (e.g., VWR 20170-010) or 96-well PCR reaction plates with 8-cap strips
Racks for 1.5 ml microcentrifuge tubes and 96-well 0.2 ml PCR reaction tubes
Cooler rack for 1.5 microcentrifuge tubes and 96-well 0.2 ml PCR reaction tubes
Acceptable surface decontaminants, for example: 10% bleach (1:10 dilution of commercial 5.25-6.0% sodium hypochlorite)
Laboratory marking pen
Appropriate disposal containers
White paper or light background for optimal visualization of colorimetric reaction (e.g., typical printer paper)
Micropipettes (2 or 10 μl, 200 μl and 1,000 μl), Multichannel Micropipettes (5-50 μl)
-20°C Freezer (frost-free or nonfrost), 4°C Refrigerator
Thermocycler, heat block or device that can be set to 65°C
PCR Work Station [UV lamp; Laminar flow (Class 100 HEPA filtered)], Vortex Mixer, Tabletop Microcentrifuge