Product Class: Other

Gel Loading Dye, Orange (6X)

Product Introduction

Gel Loading Dye, Orange (6X) is an Orange G-based loading dye for convenient gel loading and sharp bands

  • For use with agarose and non-denaturing polyacrylamide gels
  • Contains SDS for improved band sharpness
  • Contains EDTA to stop enzymatic reactions
  • Orange G will not appear in gel photographs
  • You may also be interested in our Gel Loading Dye, Purple (6X) with and without SDS, for sharp bands and no UV shadow.
Bioz Badge Exists : True
Catalog # Size Concentration
B7022S 4 ml 6 X

Product Information

Description

Gel Loading Dye, Orange (6X) is a pre-mixed loading buffer with a tracking dye for agarose and non-denaturing polyacrylamide gel electrophoresis. This solution contains SDS, which often results in sharper bands, as some restriction enzymes are known to remain bound to their DNA substrates following cleavage. EDTA is also included to chelate magnesium (up to 10  mM) in enzymatic reactions, thereby stopping the reaction. Orange G will not appear in gel photographs, and runs ahead of all but the smallest restriction fragments. Orange G migrates at approximately 50 bp on a standard 1% TBE agarose gel. This product is packaged as 4x1 ml vials.
This product is related to the following categories:
Gel Loading Buffers,
Buffers,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

1X Buffer Components

2.5% Ficoll®-400
11 mM EDTA
3.3 mM Tris-HCl
0.017% SDS
0.15% Orange G
pH 8@25°C

Product Notes

  1. Use 5 µl of Gel Loading Dye, Orange (6X) per 25 µl reaction, or 10 µl per 50 µl reaction. Mix well before loading gel.

FAQs & Troubleshooting

FAQs

  1. Are Gel Loading Dye, Blue (6x) and Gel Loading Dye Orange (6x) compatible with other systems such as SYBR (add registered mark symbol) and GelRed during gel electrophoresis?
  2. How are the 6x loading dyes packaged?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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