Product Class: Other

StickTogether™ DNA Ligase Buffer

Product Introduction

  • StickTogether™ Buffer is supplied with T3 (NEB #M0317), T7 (NEB #M0318), Hi-T4™ (NEB #M2622) and Salt-T4® (NEB #M0467) DNA Ligases    
  • At a 1X concentration, this reaction buffer ensures optimal activity of these DNA ligases
  • Provided as a 2X solution
  • Contains 2 vials of buffer
 
Bioz Badge Exists : True
Catalog # Size Concentration
B0535S 2 ml 2 X

Product Information

Description

New England Biolabs supplies a reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of T3 (NEB #M0317), T7 (NEB #M0318), Hi-T4™ (NEB #M2622) and Salt-T4® (NEB #M0467) DNA Ligases .
This product is related to the following categories:
DNA Ligases,
Buffers,
This product can be used in the following applications:
Cloning Ligation

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

1X Buffer Components

66 mM Tris-HCl
10 mM MgCl2
1 mM ATP
1 mM DTT
7.5% Polyethylene glycol (PEG 6000)
pH 7.6@25°C

Tools & Resources

Web Tools

FAQs & Troubleshooting

FAQs

  1. What is supplied in the StickTogether™ DNA Ligase Buffer pack?
  2. The StickTogether™ DNA Ligase Buffer arrived thawed.  Are the buffer and enzyme still active?
  3. Should I aliquot the StickTogether™ DNA Ligase Buffer and store it at -80°C for long-term storage?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.