Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of pUC19 DNA in 1 hour at 55°C in a total reaction volume of 50 µl. Reaction Conditions
1X NEBuffer™ r2.1
Incubate at 55°C
1X NEBuffer™ r2.1
50 mM NaCl
10 mM Tris-HCl
10 mM MgCl2
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)
Activity in NEBuffers
NEBuffer™ r1.1: 10%
NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: 50%
rCutSmart™ Buffer: 50%
Diluent Compatibility
Storage Buffer
10 mM Tris-HCl
50 mM NaCl
0.1 mM EDTA
1 mM DTT
50% Glycerol
200 µg/ml Recombinant Albumin
pH 7.4 @ 25°C
Heat Inactivation
80°C for 20 minutes Methylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive
Isoschizomers
CviAII
FaeI
Hin1II
Hsp92II
NlaIII
Activity at Temperature
@37°C: 100%