Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 25°C in a total reaction volume of 50 µl. Reaction Conditions
1X NEBuffer™ r3.1
Incubate at 25°C
1X NEBuffer™ r3.1
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl2
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)
Activity in NEBuffers
NEBuffer™ r1.1: 75%
NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: 100%
rCutSmart™ Buffer: 75%
Diluent Compatibility
Storage Buffer
10 mM Tris-HCl
250 mM NaCl
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
1 mM DTT
0.15% Triton® X-100
pH 7.4 @ 25°C
Heat Inactivation
No Methylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive
Isoschizomers
AfaI
Csp6I
RsaI
RsaNI
Activity at Temperature
@37°C: 25%