Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of T7 DNA in 1 hour at 75°C in a total reaction volume of 50 µl. Reaction Conditions
1X rCutSmart™ Buffer
Incubate at 75°C
1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)
Activity in NEBuffers
NEBuffer™ r1.1: 25%
NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: 50%
rCutSmart™ Buffer: 100%
Diluent Compatibility
Storage Buffer
10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
200 µg/ml BSA
pH 7.4 @ 25°C
Heat Inactivation
No Methylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Blocked
CpG Methylation: Not Sensitive
Isoschizomers
AjnI
BciT130I
BseBI
Bst2UI
BstNI
EcoRII
MvaI
Psp6I
Activity at Temperature
@37°C: 25%