Product Class: Other

SNAP-tag® Purified Protein

Product Introduction

The SNAP-tag® is a small protein based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT).

  • The purified SNAP-tag protein is supplied at 1 mg/ml (50 μM) concentration in 1X PBS solution containing 1 mM DTT at 1 mg/ml (50 μM)
  • Mass spectrometry analysis indicates the SNAP-tag purified protein has two species with molecular weights of 19,694 and 19,829 (with a C-terminal DTT moiety attached)
  • SNAP-tag Purified Protein can be used as a positive control for in vitro labeling with various SNAP-tag fluorescent substrates
Bioz Badge Exists : True
Catalog # Size Concentration
P9312S 50 µg 50 µM

Product Information

Description

The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

SNAP-tag Purified Protein can be used as a positive control for in vitro labeling with various SNAP-tag fluorescent substrates. The coding sequence of SNAP-tag was cloned into a pTXB1 derived E. coli T7 expression vector. SNAP-tag protein (MW: 19,694) was expressed and purified according to the instructions in the IMPACT™ kit manual (NEB #E6901). The purified SNAP-tag protein was dialyzed into 1X phosphate buffered saline (PBS) solution containing 1 mM DTT at 1 mg/ml (50 μM) and stored at -80°C.

Mass spectrometry analysis indicates the SNAP-tag purified protein has two species with molecular weights of 19,694 and 19,829 (with a C-terminal DTT moiety attached).

 

Fluorescently-labeled SNAP-tag Purified Protein

Gel showing SNAP bands on gel

Typical SDS-PAGE of SNAP-tag Purified Protein labeled with SNAP-Vista Green and visualized using a gel scanner (Typhoon 9400).
This product is related to the following categories:
Snap Tag Other,
Protein Labeling,
This product can be used in the following applications:
SNAP Companion,
Protein Labeling Snap Clip,
Cell Imaging

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Materials Required but not Supplied

  • SNAP-tag substrate, (e.g. fluorophores, oligos, affinity molecules)

Product Notes

  1. Storage: The SNAP-tag Purified Protein may be stored at -20°C for short term use, but should be kept at -80°C for long term storage. SNAP-tag Purified Protein is supplied at a concentration of 50 μM and is supplied in 1X PBS with 1mM DTT. Thaw and aliquot just before use.
  2. Fresh DTT should be added before use each time.

FAQs & Troubleshooting

FAQs

  1. Cellular Imaging and Analysis FAQs

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

Cellular Imaging and Analysis (i.e., SNAP and CLIP products)

Notice To Buyer/User: The Buyer/User has a non-exclusive license to use this system or any component thereof for RESEARCH AND DEVELOPMENT PURPOSES ONLY. Commercial use of this system or any components thereof requires a license from New England Biolabs, Inc., 240 County Road, Ipswich, MA 01938.

These patents and patent applications are owned by Covalys, or owned by the Ecole Polytechnique Fédérale de Lausanne (EPFL) and exclusively licensed to Covalys and NEB.

The products and/or their use may be covered by one or more of the following patents and patent applications:
Methods for Using O6-Alkylguanine-DNA-Alkyltransferases: US 7,939,284; US 8,367,361; EP 1 410 023; EP 2 037 271; EP 1 696 234; EP 2 211 177; JP 4195815; JP 4226053; CN 1295510; CN 1975422; and SG 100125.

Mutants of O6-Alkylguanine-DNA-Alkyltransferases: US 7,888,090. EP 1720981 (pending). 

Other Products You May Be Interested In