Product Class: Other

Furin
recombinant

P8077_cutsite

Product Introduction

Furin is a recombinant, ubiquitous subtilisin-like proprotein convertase with a minimal cleavage site of Arg-X-X-Arg˅. However, the enzyme prefers the site Arg-X-Lys/Arg-Arg˅. 

  • It is the major processing enzyme of the secretory pathway and is localized in the trans-golgi network
  • Substrates of Furin include blood clotting factors, serum proteins and growth factor receptors such as the insulin-like growth factor receptor
Bioz Badge Exists : True
Catalog # Size Concentration
P8077S 50 units 2000 units/ml
P8077L 250 units 2000 units/ml

Product Information

Description

Furin is a ubiquitous subtilisin-like proprotein convertase. It is the major processing enzyme of the secretory pathway and is localized in the trans-golgi network (1,2). Substrates of Furin include blood clotting factors, serum proteins and growth factor receptors such as the insulin-like growth factor receptor. The minimal cleavage site is Arg-X-X-Arg'. However, the enzyme prefers the site Arg-X-(Lys/Arg)-Arg'. An additional arginine at the P6 position appears to enhance cleavage (3). Furin is inhibited by EGTA, α1- Antitrypsin Portland (4) and polyarginine compounds (5).

Product Source

Isolated from Spodoptera frugiperda (Sf9) cells infected with recombinant baculovirus carrying truncated human furin.
This product is related to the following categories:
Proteases
This product can be used in the following applications:
Fusion Protein Cleavage,
Protein Purification,
Protein Digestion

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

1 unit is defined as the amount of enzyme required to cleave 25 µg of a MBP-FN-paramyosin-ΔSal substrate to 95% completion in 6 hours at 25°C in a total reaction volume of 25 µl.

Storage Buffer

10 mM MES
1 mM CaCl2
50% Glycerol
pH 7 @ 25°C

Molecular Weight

Theoretical: 52.7 kDa

Unit Assay Conditions

Two fold dilutions of Furin are incubated with 25 µg MBP-FN-paramyosin-ΔSal substrate in 20 mM HEPES, 0.1% Triton X-100, 1 mM CaCl2, 0.2mM β-mercaptoenthanol (pH 7.5 @ 25°C) in a 25 µl reaction. The reaction mix is incubated at 25°C for 6 hours. Separation of reaction products are visualized by SDS-PAGE.

Product Notes

  1. Both Furin and Onchocerca volvulus Blisterase will cleave peptide substrates with the sequence, Arg-X- (Lys/Arg)-Arg. However, the ability of either enzyme to cleave a particular protein substrate depends on its tertiary structure as well as on the amino acids immediately surrounding the cleavage site (6).

References

  1. van den Ouweland, A.M.W. et al. (1990). Nucl. Acids Res. 18, 664.
  2. Steiner, D.F. (1998). Curr. Opin. Chem. Biol.. 2,
  3. Krysan, D.J. et al. (1999). J. Biol. Chem.. 274, 23229-23234.
  4. Jean, F. et al. (1998). Proc. Natl. Acad. Sci. USA. 95, 7293-7298.
  5. Cameron, A. et al. (2000). J. Biol. Chem.. 275, 36741-36749.
  6. Poole, C.B. et al. (2003). J. Biol. Chem.. 278, 36183-36190.

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Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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