Product Class: Other

Histone H10 Human, Recombinant
cloned at NEB recombinant

  • Catalog # M2501 was discontinued on December 18, 2023

Product Introduction

Histone H10 is a non-replication-dependent histone that is highly expressed in terminally differentiated cells (3).

  • Highly pure, recombinant protein
  • Identity confirmed using Edman Degradation to sequence intact protein
  • Mass confirmed by ESI-TOF MS
  • Tested for exonuclease, endonuclease and protease contamination
Bioz Badge Exists : True

Product Information

Description

Histone H1 acts on the linker region of polynucleosome DNA to condense the chromatin into structures of ~30 nm (1). It is not necessary for octamer or nucleosome core particle formation. Eight different Histone H1 proteins have been identified in the human genome (2). Histone H10 is a non replication-dependent histone that is highly expressed in cells that have terminally differentiated (3).

Protein Sequence:
TENST SAPAA KPKRA KASKK STDHP KYSDM IVAAI QAEKN RAGSS RQSIQ KYIKS HYKVG ENADS QIKLS IKRLV TTGVL KQTKG VGASG SFRLA KSDEP KKSVA FKKTK KEIKK VATPK KASKP KKAAS KAPTK KPKAT PVKKA KKKLA ATPKK AKKPK TVKAK PVKAS KPKKA KPVKP KAKSS AKRAG KKK (Genbank accession number: P07305)


ESI-TOF Mass Spectrometry Analysis of Histone H10 Human, Recombinant
ESI-TOF Analysis of Histone H10 Human, Recombinant.


SDS-PAGE analysis of Histone H10 Human, Recombinant
SDS-PAGE analysis of Histone H10 Human, Recombinant

Lane 1 and 7: NEB Protein Ladder (NEB #P7703), Lanes 2 thru 6: 0.5, 1.0, 2.0, 5.0, 10.0 µg Histone H10 Human, Recombinant.

Product Source

An E. coli strain that carries a plasmid encoding the human histone H1 gene, H1F0 or H1FV. (Genbank accession number: X03473)
This product is related to the following categories:

Properties & Usage

Storage Buffer

20 mM Sodium Phosphate
300 mM NaCl
1 mM EDTA
pH 7 @ 25°C

Molecular Weight

Theoretical: 20732 daltons

Product Notes

  1. 1 mg/ml, 48 µM iscalculated using the molar extinction coefficient for Histone H1 (3840) and itsabsorbance at 280 nm (4,5).  1.0 A280 units = 5.4mg/ml.
  2. There is a small percentage of Histone H10 with a mass of 20863.27 which is a +131 Da difference from the major species. This correlates to Histone H10 with an intact N-terminal methionine (6).
  3. Synonyms: Histone H1.0, Histone H1(0), Histone H1'

References

  1. van Holde, K.E. (1989). Chromatin. 1-497,
  2. Marzluff, W.F., et al. (2002). Genomics. 80, 487-497.
  3. Pehrson, J.R. and Cole, R.D. (1982). Biochem. 21, 456-460.
  4. Gill, S.C. and von Hippel, P.H. (1989). Anal. Biochem. . 182, 319-326.
  5. Pace, C.N. et al. (1995). Protein Science. 4, 2411-2423.
  6. Qing, X. et al. (2010). Biochemistry. 49, 5588-5599.

FAQs & Troubleshooting

FAQs

  1. Do the histones need to be reconstituted?
  2. What are the recommended histone storage conditions?
  3. Are the histones fusion proteins or tagged proteins?
  4. Can the histones be used as substrates for protein modification enzymes? Which ones?

Tech Tips

After thawing on ice, mix well by pipetting the solution up and down. Do not centrifuge.

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.