Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.
Reaction Conditions
1X LongAmp® Taq Reaction Buffer Pack
1X LongAmp® Taq Reaction Buffer Pack
60 mM Tris-SO4
20 mM (NH4)2SO4
2 mM MgSO4
3% Glycerol
0.06% IGEPAL® CA-630
0.05% Tween® 20
(pH 9.1 @ 25°C)
Storage Buffer
10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
0.5% Tween® 20
0.5% IGEPAL® CA-630
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
No 5' - 3' Exonuclease
Yes 3' - 5' Exonuclease
Yes Strand Displacement
+ Unit Assay Conditions
1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.