Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 15 nmol dNTP into acid insoluble material in 30 minutes at 75°C. Reaction Conditions
1X EpiMark® Hot Start Taq Reaction Buffer Pack
1X EpiMark® Hot Start Taq Reaction Buffer Pack
20 mM Tris-HCl
1.8 mM MgCl2
22 mM KCl
0.06% IGEPAL® CA-630
0.05% Tween® 20
22 mM NH4Cl
(pH 8.9 @ 25°C)
Storage Buffer
10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
0.5% Tween® 20
0.5% IGEPAL® CA-630
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
No Molecular Weight
Theoretical: 94000 daltons
5' - 3' Exonuclease
Yes 3' - 5' Exonuclease
No Resulting Ends
Unit Assay Conditions
1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.