Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to excizse 1 pmol of deoxyuracil from a 34 mer oligonucleotide duplex containing a single dU site in a total reaction volume of 10 µl in 1 hour at 37°C. Reaction Conditions
1X NEBuffer™ 1
Supplement with 100 µg/ml Recombinant Albumin, Molecular Biology Grade
Incubate at 37°C
1X NEBuffer™ 1
10 mM Bis-Tris-Propane-HCl
10 mM MgCl2
1 mM DTT
(pH 7 @ 25°C)
Storage Buffer
10 mM Tris-HCl
250 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml Recombinant Albumin
50% Glycerol
0.15% Triton® X-100
pH 7.4 @ 25°C
Heat Inactivation
65°C for 20 minutes Molecular Weight
Theoretical: 29861 daltons
Unit Assay Conditions
1X NEBuffer 1 supplemented with 100 µg/ml Recombinant Albumin containing 5 pmol of fluorescently labeled oligonucleotide duplex in a total reaction volume of 10 µl. After release of dU, the oligonucleotide is cleaved by treating the resulting AP site with 100 mM NaOH for 10 minutes at 80°C.