Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C. Reaction Conditions
1X Standard Taq (Mg-free) Reaction Buffer Pack
Supplement with 1.5 mM MgCl2 solution
1X Standard Taq (Mg-free) Reaction Buffer Pack
10 mM Tris-HCl
50 mM KCl
(pH 8.3 @ 25°C)
Storage Buffer
10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.5% Tween® 20
0.5% IGEPAL® CA-630
pH 7.4 @ 25°C
Heat Inactivation
No Molecular Weight
Theoretical: 94000 daltons
5' - 3' Exonuclease
Yes 3' - 5' Exonuclease
No Strand Displacement
+ Resulting Ends
Unit Assay Conditions
1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA. Error Rate
~ 285x10-6bases