Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol dTTP into acid-insoluble material in a total reaction volume of 50ul in 1 hour at 37°C using d(A)18 as primer. Reaction Conditions
1X Terminal Transferase Reaction Buffer Pack
Supplement with 0.25 mM CoCl2
Incubate at 37°C
1X Terminal Transferase Reaction Buffer Pack
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
(pH 7.9 @ 25°C)
Storage Buffer
50 mM KPO4
100 mM NaCl
1.43 mM β-ME
50% Glycerol
0.1% Triton® X-100
pH 7.3 @ 25°C
Heat Inactivation
75°C for 20 minutes Molecular Weight
Theoretical: 58000 daltons
5' - 3' Exonuclease
No 3' - 5' Exonuclease
No Strand Displacement
No Unit Assay Conditions
1X Terminal Transferase Reaction Buffer, 0.72 μM d(A)18, 0.2 mM dTTP, and 1 μCi [3H]- dTTP in a 50 μl total reaction volume.