Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to create an AP site from 1 pmol of a 34-mer oligonucleotide duplex containing a single deoxyinosine site in a total reaction volume of 10 µl in 1 hour at 37°C. Reaction Conditions
1X ThermoPol® Reaction Buffer
Incubate at 37°C
1X ThermoPol® Reaction Buffer
20 mM Tris-HCl
10 mM (NH4)2SO4
10 mM KCl
2 mM MgSO4
0.1% Triton® X-100
(pH 8.8 @ 25°C)
Storage Buffer
10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.5% Tween® 20
0.5% IGEPAL® CA-630
pH 7.4 @ 25°C
Heat Inactivation
65°C for 20 minutes Molecular Weight
Theoretical: 25752 daltons
Unit Assay Conditions
1X ThermoPol Buffer containing 5 pmol of fluorescently labeled oligonucleotide duplex in a total reaction volume of 10 µl.