Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
Reaction Conditions
1X ThermoPol® Reaction Buffer
1X ThermoPol® Reaction Buffer
20 mM Tris-HCl
10 mM (NH4)2SO4
10 mM KCl
2 mM MgSO4
0.1% Triton® X-100
(pH 8.8 @ 25°C)
Storage Buffer
10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
0.5% Tween® 20
0.5% IGEPAL® CA-630
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
No Molecular Weight
Theoretical: 94000 daltons
5' - 3' Exonuclease
Yes 3' - 5' Exonuclease
No Strand Displacement
+ Resulting Ends
Unit Assay Conditions
1X ThermoPol Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA. Error Rate
~ 285x10-6bases