Product Class: Kit

BioBrick® Assembly Kit

This product was discontinued on 12/15/2021. 

Individual components can be purchased separately. Please contact info@neb.com.

We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. All information on the website has been updated to reflect this change. Find more details at www.neb.com/BSA-free.

  • Catalog # E0546 was discontinued on December 15, 2021

Product Introduction

  • Assemble multi-compontent genetic systems using BioBrick parts 
  • BioBricks are DNA sequences that encode a defined biological function 
  • Easily assemble any two BioBrick parts using the same protocol
Bioz Badge Exists : True
Catalog # Size Concentration

Product Information

Description

The BioBrick® Assembly Kit provides a streamlined method for assembly of BioBrick parts into multi-component genetic systems. BioBrick parts are DNA sequences that encode a defined biological function and can be readily assembled with any other BioBrick part. The process for assembling any two BioBrick parts is identical and results in a new composite BioBrick part.

The BioBrick Assembly Kit contains EcoRI-HF™, XbaI, SpeI, PstI, T4 DNA Ligase and NEBuffer 2.1.

BioBrick Assembly Overview BioBrick Assembly Overview
This product is related to the following categories:
Discontinued (<3 years)
This product can be used in the following applications:
BioBrick® Assembly

Kit Components

Kit Components

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Protocols, Manuals & Usage

Protocols

  1. Digestion Protocol for BioBrick Assembly Kit (E0546)
  2. Ligation Protocol using BioBrick Assembly Kit® (E0546)

FAQs & Troubleshooting

FAQs

  1. I am competing in the iGEM competition. Do you have any products that I should consider from NEB?
  2. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

The BioBrick® Assembly Kit was developed in partnership with Ginkgo BioWorks. For more details and for technical questions, please see: http://ginkgobioworks.com/support.

BioBrick is a trademark of The BioBricks Foundation. (http://www.biobricks.org)

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