Product Class: Other

Diluent B

This product has been discontinued and replaced with Diluent B (with rAlbumin).

  • Catalog # B8002 was discontinued on October 10, 2023

Product Introduction

  • Diluent B is recommended for making dilutions of New England Biolabs, Inc. restriction enzymes. 
  • Contains 4 x 1.3 ml vials.
  • When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml.
  • Restriction enzymes should be stored at their selling concentrations.
Bioz Badge Exists : True
Catalog # Size Concentration

Product Information

Description
This product is related to the following categories:

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

1X Buffer Components

300 mM NaCl
10 mM Tris-HCl
1 mM DTT
0.1 mM EDTA
500 µg/ml BSA
50% Glycerol
pH 7.4@25°C

Product Notes

  1. These enzyme diluent buffers are not for use with VentR® DNA Polymerases. For diluting VentR® DNA Polymerases, use (NEB #B8004S).

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. What is the composition of Diluent Buffer B?
  2. How long can the diluted enzyme be stored?
  3. Why is my Restriction Enzyme not cutting DNA?
  4. Why do I see additional DNA bands on my gel after a restriction digest?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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