Product Class: Other

CutSmart® Buffer

This product has been replaced by rCutSmart Buffer (NEB #B6004).

  • Catalog # B7204 was discontinued on December 15, 2021

Product Introduction

  • Over 210 enzymes are 100% active in CutSmart® Buffer simplifying double digests
  • Visit NEBCutSmart.com to learn more about the CutSmart Buffer system
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Product Information

Description

New England Biolabs provides a colorcoded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer.

Over 215 restriction enzymes are 100% active in CutSmart Buffer, making it significantly easier to set up your double digest reactions. Since CutSmart Buffer includes BSA, there are also fewer tubes and pipetting steps to worry about. Additionally, many DNA modifying enzymes are 100% active in CutSmart Buffer, eliminating the need for subsequent purification.

This product is related to the following categories:

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

1X Buffer Components

50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml BSA
pH 7.9@25°C

Product Notes

  1. The pH of 10X CutSmart Buffer is between pH 7.8 and 8.0.

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. You have replaced NEBuffer 1 with NEbuffer 1.1, NEBuffer 2 with NEBuffer 2.1, and NEBuffer 3 with 3.1. Where is NEBuffer 4.1?
  2. Why did you add BSA into all the restriction enzyme reaction buffers?
  3. Why did you remove DTT from your restriction enzyme buffers?
  4. I don’t want to use BSA in my buffer. What are my options?
  5. Which restriction enzymes are used in GoldenBraid Assembly?
  6. Are the alkaline phosphatases active in NEBuffers?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Trademarks

CUTSMART™ is a trademark of New England Biolabs, Inc.

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