Description: New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer.
\nThe NEBuffer Sets contain multiple vials of buffers, which are indicated in the product name. NEBuffer Set (r1.1, r2.1, r3.1 and rCutSmart) is formulated with Recombinant Albumin.\n
\nNuclease-free Water is ideal for the preparation of reagents and for use in enzymatic reactions. No toxic agents, such as DEPC, are used in the production of this water, so as to avoid inhibition in enzymatic reactions.\n
\nReaction Buffer Compositions: See Performance Chart for Restriction Enzymes or visit www.neb.com for details.
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\n"},{"SortOrder":7,"Sku":"B7006","ProductName":"NEBuffer™ Set (EcoRI/SspI, DpnII)"},{"SortOrder":8,"Sku":"B1500","ProductName":"Nuclease-free Water"},{"SortOrder":9,"Sku":"B6002","ProductName":"NEBuffer™ r2.1"},{"SortOrder":10,"Sku":"B6003","ProductName":"NEBuffer™ r3.1"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":6,"Category":"Diluent Buffers","ProductFamily":"1","ProductFamilyDescription":"
Description: Diluent Buffers are recommended for making dilutions of restriction endonucleases. When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml. Diluent preference for each restriction endonuclease is listed with its catalog entry.
\nStorage Conditions: Store at –20°C.
\nDiluent Buffer Compositions: See Performance Chart for Restriction Enzymes or visit www.neb.com for details.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"B8532","ProductName":"Diluent A (with rAlbumin)"},{"SortOrder":2,"Sku":"B8533","ProductName":"Diluent B (with rAlbumin)"},{"SortOrder":3,"Sku":"B8534","ProductName":"Diluent C (with rAlbumin)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":7,"Category":"Gel Loading Dyes","ProductFamily":"1","ProductFamilyDescription":"Description: Pre-mixed loading dye solutions are available with a choice of tracking dyes, for agarose and non-denaturing polyacrylamide gel electrophoresis. Three of the solutions contain SDS, which often results in sharper bands, as some restriction enzymes remain bound to their DNA substrates following cleavage. Some interference may be observed when using SYBR® or GelRed® as precast dyes in the presence of increased concentrations of SDS. When using these dyes as precast dyes, NEB recommends using our Gel Loading Dye, Purple (6X), no SDS (NEB #B7025). Gel Loading Dyes contain EDTA to chelate up to 10 mM magnesium, thereby stopping the reaction. Bromophenol blue is the standard tracking dye for electrophoresis. It migrates at approximately 300 bp on a standard 1% TBE agarose gel. Orange G will not appear in gel photographs, and runs ahead of all but the smallest restriction fragments, migrating at approximately 50 bp on a standard 1% TBE agarose gel. NEB also offers a unique purple dye which migrates similarly to Bromophenol blue. Specifically chosen, this dye does not leave a shadow under UV light.
\nGel Loading Dye Compositions: Visit www.neb.com for details.
\nNote: Use 5 µl of Gel Loading Dye per 25 µl reaction, or 10 µl per 50 µl reaction. Mix well before loading gel. Store at room temperature.
\nDescription: Q5 High-Fidelity DNA Polymerase sets the standard for performance, ultra-low error rates and fidelity (~ 280 times higher than Taq). Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability.
\nThe Q5 buffer system provides superior performance with minimal optimization across a broad range of amplicons, regardless of GC content. For routine or complex amplicons up to ~65% GC content, Q5 Reaction Buffer provides reliable and robust amplification. For amplicons with high GC content ( >65% GC), addition of the Q5 High GC Enhancer ensures continued maximum performance.
\nQ5 Hot Start DNA Polymerase: In contrast to chemically-modified or antibody-based hot start polymerases, NEB’s Q5 Hot Start utilizes a unique synthetic aptamer. This structure binds to the polymerase through non-covalent interactions, blocking activity during the reaction setup. The polymerase is activated during normal cycling conditions, allowing reactions to be set up at room temperature. Q5 Hot Start does not require a separate high temperature activation step, reducing the potential for sample degradation, shortening reaction times and increasing ease-of-use. Q5 Hot Start is an ideal choice for high specificity amplification and provides robust amplification of a wide variety of amplicons, regardless of GC content.
\nQ5U Hot Start High-Fidelity DNA Polymerase: A modified version of Q5 High-Fidelity DNA Polymerase capable of incorporating dUTP for carryover prevention. Q5U is also compatible with USER cloning methods and enables the amplification of bisulfite treated/ deaminated DNA.
\nQ5 Blood Direct 2X Master Mix: Amplify a wide variety of targets direct from dried blood spots or up to 30% whole human blood with this unique master mix.
\nAdditional Formats: For added convenience, Q5 DNA Polymerase is available in master mix format or as a kit. Master mix formulations include dNTPs, Mg++ and all necessary buffer components. The Q5 High-Fidelity PCR Kit contains the Q5 High-Fidelity 2X Master Mix, nuclease-free water and the Quick-Load Purple 1 kb Plus DNA Ladder. The Q5 Site-Directed Mutagenesis Kit, with or without competent cells, is also available.
\nConcentration: 2,000 units/ml
\nVisit Q5PCR.com for more information.
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Description: DNA polymerases with high fidelity are important for applications in which the DNA sequence needs to be correct after amplification. Manufactured and quality controlled at New England Biolabs, Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion Hot Start Flex DNA Polymerase is available as a standalone enzyme or in a master mix format, and enables high specificity amplification. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long amplicons.
\nAdditional Formats: Phusion and Phusion Hot Start Flex DNA Polymerases are also available in master mix format. Phusion master mixes are available with HF or GC Buffer. The Phusion PCR Kit contains Phusion Polymerase, Phusion HF and GC buffers, deoxynucleotides, MgCl2, DMSO and DNA size standards.
\nConcentration: 2,000 units/ml
\n* Phusion DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. Phusion™ is a registered trademark and property of Thermo Fisher Scientific.
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\nOneTaq Hot Start DNA Polymerase: OneTaq Hot Start DNA Polymerase utilizes an aptamer-based inhibitor. The hot start formulation combines convenience with decreased interference from primer-dimers and secondary products.
\nThe aptamer-based inhibitor binds reversibly, blocking polymerase activity at temperatures below 45°C, allowing reactions to be set up at room temperature. OneTaq Hot Start DNA Polymerase is activated during normal cycling conditions, eliminating the need for a separate high temperature incubation step to activate the enzyme. OneTaq Hot Start DNA Polymerase can therefore be substituted into typical or existing Taq-based protocols.
\nOneTaq and OneTaq Hot Start are provided with Standard Reaction Buffer, GC Reaction Buffer and High GC Enhancer. Recommendations for buffer selection, based on % GC content, are shown below.
\nQuick-Load formats offer direct loading of PCR products onto gels, eliminating the need to add a separate loading/tracking dye.
\nAdditional Formats: For added convenience, OneTaq and OneTaq Hot Start DNA Polymerases are available in master mix format. Master mixes are available with Standard or GC Buffer. High GC Enhancer is also provided with master mixes containing GC Buffer. For direct gel loading, Quick-Load versions of master mixes are also available. OneTaq® RT-PCR Kit combines two powerful mixes, M-MuLV Enzyme Mix and OneTaq Hot Start 2X Master Mix with Standard Buffer for 2-step RT-PCR applications. The OneTaq One-Step RT-PCR Kit offers sensitive and robust end-point detection of RNA templates. cDNA synthesis and PCR amplification steps are performed in a single reaction using gene-specific primers, resulting in a streamlined RT-PCR protocol.
\nConcentration: 5,000 units/ml
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\nHot Start Taq DNA Polymerase: With value pricing and attractive commercial terms, Hot Start Taq is an ideal choice for research applications. In contrast to chemically modified or antibody-based hot start polymerases, NEB’s Hot Start Taq utilizes an aptamer-based technology. The unique aptamer binds to the polymerase through non-covalent interactions, inhibiting polymerization at non-permissive temperatures. This novel method eliminates the need for an activation step, reducing the potential for sample degradation and decreasing overall reaction time.
\nAdditional Formats: For added convenience, Taq and Hot Start Taq DNA Polymerase are available in master mix format. For direct gel loading, a Quick-Load version of the Taq 2X Master Mix is also available. The Taq PCR Kit contains Taq DNA Polymerase, dNTP Mix, Buffer, MgCl2 and the Quick-Load Purple 1 kb Plus DNA Ladder. The Multiplex PCR 5X Master Mix formulation has been specifically optimized for enhanced performance in multiplex PCR reactions.
\nConcentration: 5,000 units/ml
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\nLongAmp Hot Start Taq DNA Polymerase: LongAmp Hot Start Taq DNA Polymerase utilizes a unique synthetic aptamer. This structure binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal PCR cycling conditions.
\nAdditional Formats: For added convenience, LongAmp Taq and LongAmp Hot Start Taq are available in master mix format. The LongAmp Taq PCR Kit includes LongAmp Taq DNA Polymerase (2,500 units/ml), dNTP Mix (10 mM), LongAmp Taq Reaction Buffer Pack (5X), MgSO4 (100 mM) and nuclease-free water.
\nConcentration: 2,500 units/ml
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\nDeep Vent DNA Polymerase is a modified version of Vent DNA Polymerase. Deep Vent has similar fidelity with even more stability than Vent at temperatures of 95 to 100°C, with a half-life of 8 hours at 100°C.
\nVent (exo– ) DNA Polymerase has been genetically engineered to eliminate the 3´→ 5´ proofreading exonuclease activity associated with Vent DNA Polymerase. The fidelity of polymerization by this form is reduced to a level about 2-fold higher than that of Taq DNA Polymerase. Likewise, Deep Vent (exo– ) DNA Polymerase has been genetically engineered to eliminate the 3´→ 5´ proofreading exonuclease activity associated with Deep Vent DNA Polymerase.
\nSource: Vent DNA Polymerase is purified from a strain of E. coli that carries the Vent DNA Polymerase gene from the archaea Thermococcus litoralis. Vent (exo– ) is purified from an E. coli strain that carries the Vent (D141A/E143A) DNA Polymerase gene, a genetically engineered form of the native DNA polymerase.
\nDeep Vent DNA Polymerase is purified from a strain of E. coli that carries the Deep Vent DNA Polymerase gene from Pyrococcus species GB-D. Deep Vent (exo– ) is purified from an E. coli strain that carries the Deep Vent (D141A/E143A) DNA Polymerase gene, a genetically engineered form of the native polymerase.
\nConcentration: 2,000 units/ml
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\nThe Luna Universal Probe qPCR Master Mix is a 2X reaction mix optimized for real-time qPCR detection and quantitation of target DNA sequences using hydrolysis probes.
\nEach Hot Start Taq-based Luna qPCR master mix has been formulated with a unique passive reference dye that is compatible across a wide variety of instrument platforms, including those that require a ROX reference signal. This means that no additional components are required to ensure machine compatibility. The mixes also contain dUTP, enabling carryover prevention when reactions are treated with Antarctic Thermolabile UDG. A blue visible dye assists in tracking the reagents when pipetting into clear, multi-welled PCR plates. These features, combined with rapid, sensitive and precise real-time qPCR performance, make Luna the universal choice for all your qPCR experiments.
","ProductFamilyIconCharacters":["n","r","p","h","9"],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"M3003","ProductName":"Luna® Universal qPCR Master Mix"},{"SortOrder":2,"Sku":"M3004","ProductName":"Luna® Universal Probe qPCR Master Mix"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":2,"Category":"LunaScript® RT SuperMix, SuperMix Kit & Master Mix Kit (Primer-free)","ProductFamily":"1","ProductFamilyDescription":"Description: The LunaScript RT SuperMix Kit is an optimized master mix containing all the necessary components for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow. It features the thermostable Luna Reverse Transcriptase, which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is also included to protect template RNA from degradation. LunaScript RT SuperMix Kit contains random hexamer and poly-dT primers, which allow for even coverage across the length of the RNA targets. LunaScript RT SuperMix Kit also includes a No-RT Control Mix and Nuclease-free Water.
\nLunaScript RT Master Mix Kit (Primer-free) is the same formulation as the LunaScript RT SuperMix Kit but does not contain primers. It includes all components needed for carrying out first strand cDNA synthesis except for RNA template and user-supplied primers. It provides flexible options for using different primers (dT primers, random primers, gene-specific primers, modified primers, etc.) for first strand cDNA synthesis.
\nThe LunaScript RT SuperMix Kit Includes:
\nThe LunaScript RT Master Mix Kit (Primer-free) Includes:
\nDescription: The Luna RT-qPCR kits contain a novel, in\nsilico-designed reverse transcriptase (RT) engineered for\nimproved performance. Both the Luna WarmStart Reverse\nTranscriptase and Hot Start Taq DNA Polymerase, included\nin these kits, utilize a temperature-sensitive, reversible\naptamer, which inhibits activity below 45°C. This enables\nroom temperature reaction setup and prevents undesired\nnon-specific activity. Furthermore, the WarmStart RT has\nincreased thermostability, improving performance at higher\nreaction temperatures.\n
\nThe Luna Probe One-Step RT-qPCR 4X Mix with UDG\n(NEB #M3019) supports robust, sensitive detection and\nquantitation of up to 5 targets in a multiplexed reaction.\nIt is supplied at a 4X concentration and enables higher\namounts of sample input, which is relevant for applications\nwhere RNA present in low abundance is of interest, such\nas pathogen detection. For simplified reaction setup, the\nsingle tube master mix format consolidates components\nfor the one-step RT-qPCR reaction. It also includes\ndUTP and UDG in the mix for reduced risk of carryover\ncontamination. This mix is also available without ROX (NEB #M3029) for instruments that do not require the ROX passive reference dye.
\nThe LyoPrime Luna® Probe One-Step RT-qPCR Mix with UDG (NEB #L4001) is supplied in a lyophilized format, which can be rehydrated by simply adding nuclease-free water prior to use.
\nThe Luna Universal One-Step RT-qPCR Kit (NEB #E3005) is\noptimized for dye-based real-time quantitation of target\nRNA sequences via the SYBR/FAM fluorescence channel of\nmost real-time instruments.\n
\nThe Luna Universal Probe One-Step RT-qPCR Kit (NEB #E3006) is optimized for real-time quantitation of target RNA\nsequences using hydrolysis probes.\n
\nFor instruments that do not utilize ROX normalization,\nthe Luna Probe One-Step RT-qPCR Kit (No ROX) (NEB\n#E3007) contains no reference dye. If ROX normalization is\ndesired, ROX can be added; this is only necessary with the\nE3007 product.
\nThe other Luna products contain dUTP and enable carryover prevention when reactions are treated with Antarctic Thermolabile UDG (NEB #M0372). A blue visible dye assists in tracking the reagents when pipetting into clear, multi-welled PCR plates. The reverse transcriptase, featured in the Luna One-Step RT-qPCR products is a novel, engineered WarmStart enzyme developed for robust performance and increased thermostability. These features, combined with rapid, sensitive and precise real-time qPCR performance, make Luna the universal choice for all your qPCR and RT-qPCR experiments.
\nThe Luna Probe One-Step\nRT-qPCR Kit (No ROX) Includes:
\nThe Luna Universal One-Step\nRT-qPCR Kit Includes:
\nThe Luna Universal Probe One-Step\nRT-qPCR Kit Includes:
\nDescription: The Luna Cell Ready One-Step RT-qPCR Kit provides all the necessary components for direct, dye-based RNA detection and quantitation, bypassing the need for RNA extraction and purification.
\nThe Luna Cell Ready Probe One-Step RT-qPCR Kit provides all the necessary components for direct, probe-based RNA detection and quantitation, bypassing the need for RNA extraction and purification.
\nCell cultures are often analyzed for gene expression or treatment responses as a proxy for a living organism. Traditionally, RNA is extracted and purified from treated cells via column-based or chemical methods. Coordinating the actions of DNase I and the Luna Cell Ready Protease, the Luna Cell Ready Lysis Module offers a simple alternative workflow resulting in effective cell lysis, RNA release, and genomic DNA removal simultaneously in a 15-minute protocol. The Lysis Module includes a unique Luna Cell Ready RNA Protection Reagent that maintains RNA integrity during cell lysis. The lysis capacity spans 10–100,000 cells in a 50 µl lysis reaction. Up to 2 µl of lysate (equivalent to RNA from 0.2–4,000 cells) can be transferred into 20 µl downstream RT-qPCR reactions. Similar to other Luna products, the lysis buffer includes an inert blue tracking dye for visual assistance throughout the workflow.
\nThe Luna Cell Ready One-Step RT-qPCR Kit Includes:
\nThe Luna Cell Ready Probe One-Step RT-qPCR Kit Includes:
\nWarmStart Fluorescent LAMP/RT-LAMP kits are designed to provide a simple, one-step solution for Loop Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. LAMP and RT-LAMP are commonly used isothermal techniques that provide rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase.
\nThe supplied master mixes contain an optimized blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Both enzymes have been engineered for improved performance in LAMP and RT-LAMP reactions. The inclusion of dUTP and UDG in the master mix (as noted in the product name) reduces the possibility of carryover contamination between reactions. A fluorescent dye is also supplied to enable real-time fluorescence measurement of LAMP. All WarmStart LAMP/RT-LAMP kits are compatible with multiple detection methods, including turbidity detection, real-time fluorescence detection (when used with LAMP fluorescent dye) and end-point visualization.
\nThe LyoPrime WarmStart™ Fluorescent LAMP/RT-LAMP Mix (with UDG) is supplied in a lyophilized format, which can be rehydrated by simply adding nuclease-free water prior to use.
\nThe WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG) includes:
\nThe WarmStart Fluorescent LAMP/RT-LAMP Kit includes:
\nThe LyoPrime WarmStart™ Fluorescent LAMP/RT-LAMP Mix (with UDG) includes:
\nDescription: The WarmStart Colorimetric LAMP/RT-LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visitble pH indicator for rapid and easy detection of Loop Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions. WarmStart Colorimetric LAMP/RT-LAMP 2X Master Mix (with UDG) contains dUTP and UDG in the master mix, which reduces the possibility of carryover contamination between reactions.
\nThis system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow (an overview of LAMP and primer design can be found in the Featured Videos section). This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15–40 minutes.
","ProductFamilyIconCharacters":["n","r","^","h"],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"M1800","ProductName":"WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA)"},{"SortOrder":2,"Sku":"M1804","ProductName":"WarmStart® Colorimetric LAMP 2X Master Mix with UDG"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":4,"Category":"Bst DNA Polymerases","ProductFamily":"1","ProductFamilyDescription":"Description: Bst DNA Polymerase, Large Fragment, is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5´→ 3´ polymerase activity, but lacks 5´→ 3´ exonuclease activity.
\nBst DNA Polymerase, Full Length is the full length polymerase from Bacillus stearothermophilus. It has 5´→ 3´ polymerase and double-strand specific 5´→ 3´ exonuclease activities, but lacks 3´→ 5´ exonuclease activity.
\nBst 2.0 DNA Polymerase is an in silico designed homologue of Bst DNA Polymerase, Large Fragment. It contains 5´→ 3´ DNA polymerase activity and strong strand displacement activity but lacks 5´→ 3´ exonuclease activity. It has improved amplification speed, yield, salt tolerance and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment.
\nBst 2.0 WarmStart DNA Polymerase utilizes aptamer technology to inhibit activity at non-permissive temperatures (< 50°C). Like “Hot Start” PCR polymerases, the WarmStart feature enables room temperature set up and prevents non-templated addition of nucleotides, increasing reaction efficiencies. Additionally, no separate activation step is required to release the aptamer from the enzyme. Bst 2.0 WarmStart DNA Polymerase permits reaction temperatures from 60–72°C. Bst 2.0 WarmStart DNA Polymerase is also available in a glycerol-free format to support lyophilization and automation workflows.
\nBst 3.0 DNA Polymerase is a similarly designed in silico homologue engineered and fused to a novel nucleic acid binding domain for improved isothermal amplification performance and increased reverse transcription activity. Bst 3.0 DNA Polymerase contains 5´→ 3´ DNA polymerase activity with either DNA or RNA templates but lacks 5´→ 3´ and 3´→ 5´ exonuclease activity. It demonstrates robust performance in the presence of inhibitors and significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.
\nBst-XT WarmStart DNA Polymerase combines the fast polymerization speed of Bst 3.0 and the high specificity of Bst 2.0.
\nConcentration: Bst DNA Polymerase, Full Length: 5,000 units/ml. All others: 8,000 and 120,000 units/ml. NEB #M0402 only sold at 120,000 units/ml.
\nHeat Inactivation: 80°C for 20 minutes
\nUsage Notes: No Bst DNA Polymerase-based products can be used for thermal cycle sequencing or PCR. Bst 2.0 WarmStart DNA Polymerase permits reaction temperatures from 60–72°C. Bst-XT WarmStart DNA Polymerase has optimal reaction performance from 50–70°C. Generally, reaction temperatures above 72°C are not recommended for any Bst DNA Polymerase-based product.
","ProductFamilyIconCharacters":["n","r","7","^","q"],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"M0275","ProductName":"Bst DNA Polymerase, Large Fragment"},{"SortOrder":2,"Sku":"M0328","ProductName":"Bst DNA Polymerase, Full Length"},{"SortOrder":3,"Sku":"M0537","ProductName":"Bst 2.0® DNA Polymerase"},{"SortOrder":4,"Sku":"M0538","ProductName":"Bst 2.0 WarmStart® DNA Polymerase"},{"SortOrder":5,"Sku":"M0402","ProductName":"Bst 2.0 WarmStart® DNA Polymerase (Glycerol-free)"},{"SortOrder":6,"Sku":"M0374","ProductName":"Bst 3.0® DNA Polymerase"},{"SortOrder":7,"Sku":"M9204","ProductName":"Bst-XT WarmStart™ DNA Polymerase"},{"SortOrder":8,"Sku":"M9205","ProductName":"Bst-XT WarmStart™ DNA Polymerase (Glycerol-free)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""}],"Products":{"Products":[{"SortOrder":1,"Sku":"L4401","ProductName":"LyoPrime WarmStart® Fluorescent LAMP/RT-LAMP Mix (with UDG)"},{"SortOrder":2,"Sku":"M0269","ProductName":"phi29 DNA Polymerase"},{"SortOrder":3,"Sku":"E1604","ProductName":"phi29-XT WGA Kit"},{"SortOrder":4,"Sku":"E1603","ProductName":"phi29-XT RCA Kit"},{"SortOrder":5,"Sku":"M1708","ProductName":"WarmStart® Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":8,"Category":"RT-PCR","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E1555","ProductName":"LunaScript® Multiplex One-Step RT-PCR Kit"},{"SortOrder":2,"Sku":"E5315","ProductName":"OneTaq® One-Step RT-PCR Kit"},{"SortOrder":3,"Sku":"E5310","ProductName":"OneTaq® RT-PCR Kit"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":9,"Category":"Polymerases for DNA Manipulation","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"M0309","ProductName":"PreCR® Repair Mix"},{"SortOrder":2,"Sku":"M0327","ProductName":"Sulfolobus DNA Polymerase IV"},{"SortOrder":3,"Sku":"M0261","ProductName":"Therminator™ DNA Polymerase"},{"SortOrder":4,"Sku":"M0209","ProductName":"DNA Polymerase I (E. coli)"},{"SortOrder":5,"Sku":"M0210","ProductName":"DNA Polymerase I, Large (Klenow) Fragment"},{"SortOrder":6,"Sku":"M0212","ProductName":"Klenow Fragment (3´→5´ exo-)"},{"SortOrder":7,"Sku":"M0203","ProductName":"T4 DNA Polymerase"},{"SortOrder":8,"Sku":"M0274","ProductName":"T7 DNA Polymerase (unmodified)"},{"SortOrder":9,"Sku":"M0330","ProductName":"Bsu DNA Polymerase, Large Fragment"},{"SortOrder":10,"Sku":"M0315","ProductName":"Terminal Transferase"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":10,"Category":"Nucleotide Solutions & Buffers","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"Nucleotides","ProductFamily":"1","ProductFamilyDescription":"Description:\n
\nDeoxynucleotide Solution Set:\n
\nFour separate solutions of ultrapure deoxynucleotide\n(dATP, dCTP, dGTP and dTTP). Each deoxynucleotide\nis supplied at a concentration of 100 mM.\n
Deoxynucleotide Solution Mix:\n
\nAn equimolar solution of ultrapure dATP, dCTP, dGTP\nand dTTP. Each deoxynucleotide is supplied at a concentration of 10 mM.\n
Ribonucleotide Solution Set:\n
\nFour separate solutions of ATP, CTP, GTP and UTP, pH\n7.5, as sodium salts.\n
Ribonucleotide Solution Mix:\n
\nA buffered equimolar solution of ribonucleotide triphosphates rATP, rCTP, rGTP and rUTP. Each nucleotide\nis supplied at a concentration of 25 mM (total rNTP\nconcentration equals 100 mM).\n
7-deaza-dGTP:\n
\n7-deaza contains a 5 mM solution of 7-deaza-dGTP as\na dilithium salt.
5-methyl-dCTP:
\n5mdCTP is supplied as a triethylammonium salt in Milli-Q® water.
Pseudouridine-5'-Triphosphate (Pseudo-UTP):
\nSupplied as a sodium salt, pH 7.0.
N1-Methyl-Pseudouridine-5'-Triphosphate (N1-Methyl-Pseudo-UTP):
\nSupplied as a sodium salt, pH 7.5.
5-Methyl-Citidine-5'-Triphosphate (5-Methyl-CTP):
\nSupplied as a sodium salt, pH 7.3.
5-Methoxy-Uridine-5'-Triphosphate (5-Methoxy-UTP):
\nSupplied as a sodium salt, pH 7.1.
dATP Solution:\n
\ndATP Solution contains a 100 mM solution of dATP as a\nsodium salt at pH 7.4.\n
dUTP Solution:\n
\ndUTP Solution contains a 100 mM solution of dUTP as\na sodium salt at pH 7.5.\n
dGTP Solution:\n
\ndGTP Solution contains a 100 mM solution of dGTP as\na sodium salt at pH 7.5.\n
Acyclonucleotide Set: Four separate tubes of acyNTPs (acyATP, acyCTP,\nacyGTP and acyTTP). Acyclonucleotides are supplied dry, as the triethylammonium salt. Addition of 50 µl of distilled or de-ionized\n(Milli-Q) water will result in a final concentration of\n10 mM acyNTP.
\nAcyclonucleotides (acyNTPs) act as chain\nterminators and are thus useful in applications that\nnormally employ dideoxynucleotides such as DNA\nsequencing and SNP detection. AcyNTPs are especially\nuseful in applications with archaeal DNA Polymerases,\nmore specifically with Therminator DNA Polymerase.\nTherminator DNA Polymerase is an engineered enzyme\nwith an increased capacity to incorporate analogs\nwith altered sugars, such as ribonucleotides,\ndideoxynucleotides, 2´ deoxynucleotides and especially\nacyclo-base analogs.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"N0460","ProductName":"Acyclonucleotide Set"},{"SortOrder":2,"Sku":"N0446","ProductName":"Deoxynucleotide (dNTP) Solution Set"},{"SortOrder":3,"Sku":"N0447","ProductName":"Deoxynucleotide (dNTP) Solution Mix"},{"SortOrder":4,"Sku":"N0450","ProductName":"Ribonucleotide Solution Set"},{"SortOrder":5,"Sku":"N0466","ProductName":"Ribonucleotide Solution Mix"},{"SortOrder":6,"Sku":"N0445","ProductName":"7-deaza-dGTP"},{"SortOrder":7,"Sku":"P0756","ProductName":"Adenosine 5'-Triphosphate (ATP)"},{"SortOrder":8,"Sku":"N0356","ProductName":"5-methyl-dCTP"},{"SortOrder":9,"Sku":"N0433","ProductName":"Pseudouridine-5´-Triphosphate (Pseudo-UTP)"},{"SortOrder":10,"Sku":"N0431","ProductName":"N1-Methyl-Pseudouridine-5´-Triphosphate (N1-Methyl-Pseudo-UTP)"},{"SortOrder":11,"Sku":"N0432","ProductName":"5-Methyl-Cytidine-5´-Triphosphate (5-Methyl-CTP)"},{"SortOrder":12,"Sku":"N0434","ProductName":"5-Methoxy-Uridine-5´-Triphosphate (5-Methoxy-UTP)"},{"SortOrder":13,"Sku":"N0440","ProductName":"dATP Solution"},{"SortOrder":14,"Sku":"N0459","ProductName":"dUTP Solution"},{"SortOrder":15,"Sku":"N0442","ProductName":"dGTP Solution"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":2,"Category":"Polymerase Reaction Buffers","ProductFamily":"1","ProductFamilyDescription":"Description: Q5 Reaction Buffer and High GC Enhancer are provided with both Q5 and Q5 Hot Start High-Fidelity DNA Polymerases.
\nPhusion High-Fidelity DNA Polymerase is supplied with 5X Phusion HF Buffer, 5X Phusion GC Buffer, DMSO, and 50 mM MgCl2.
\nStandard Taq Reaction Buffer is provided with Taq DNA Polymerase as an alternative to the ThermoPol Reaction Buffer.
\nThermoPol Reaction Buffer is provided with Taq, Vent, Deep Vent, Bst Full Length and Bst Large Fragment, Sulfolobus IV and Therminator DNA Polymerases; this buffer contains 2 mM MgSO4 when the buffer is diluted to its final 1X concentration.
\nIsothermal Amplification Buffer is supplied with Bst 2.0 and Bst 2.0 WarmStart DNA Polymerases.
\nIsothermal Amplification Buffer II is supplied with Bst 3.0 DNA Polymerase.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"B9027","ProductName":"Q5® Reaction Buffer Pack"},{"SortOrder":2,"Sku":"B0518","ProductName":"Phusion® HF Buffer Pack"},{"SortOrder":3,"Sku":"B0519","ProductName":"Phusion™ GC Buffer Pack"},{"SortOrder":4,"Sku":"B9014","ProductName":"Standard Taq Reaction Buffer Pack"},{"SortOrder":5,"Sku":"B9015","ProductName":"Standard Taq (Mg-free) Reaction Buffer Pack"},{"SortOrder":6,"Sku":"B9004","ProductName":"ThermoPol® Reaction Buffer Pack"},{"SortOrder":7,"Sku":"B0537","ProductName":"Isothermal Amplification Buffer Pack"},{"SortOrder":8,"Sku":"B0374","ProductName":"Isothermal Amplification Buffer II Pack"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""}],"Products":{"Products":[]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":11,"Category":"cDNA Synthesis","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"cDNA Synthesis Selection Chart","ProductFamily":"1","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E3010","ProductName":"LunaScript® RT SuperMix Kit"},{"SortOrder":2,"Sku":"E3025","ProductName":"LunaScript® RT Master Mix Kit (Primer-free)"},{"SortOrder":3,"Sku":"E6560","ProductName":"ProtoScript® II First Strand cDNA Synthesis Kit"},{"SortOrder":4,"Sku":"E6300","ProductName":"ProtoScript® First Strand cDNA Synthesis Kit"},{"SortOrder":5,"Sku":"M0466","ProductName":"Template Switching RT Enzyme Mix"},{"SortOrder":6,"Sku":"M0681","ProductName":"Induro® Reverse Transcriptase"},{"SortOrder":7,"Sku":"M0368","ProductName":"ProtoScript® II Reverse Transcriptase"},{"SortOrder":8,"Sku":"M0253","ProductName":"M-MuLV Reverse Transcriptase"},{"SortOrder":9,"Sku":"M0277","ProductName":"AMV Reverse Transcriptase"},{"SortOrder":10,"Sku":"M0380","ProductName":"WarmStart® RTx Reverse Transcriptase"},{"SortOrder":11,"Sku":"M0439","ProductName":"WarmStart® RTx Reverse Transcriptase (Glycerol-free)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"1","DisplayAsTableColumns":[{"FieldName":"FullProductName","ColumnDisplayName":"Product"},{"FieldName":"CatalogNumber","ColumnDisplayName":"NEB #"},{"FieldName":"Size","ColumnDisplayName":"Size"},{"FieldName":"CatalogFeatures","ColumnDisplayName":"Features"}],"GroupFieldName":""}],"Products":{"Products":[{"SortOrder":1,"Sku":"E3010","ProductName":"LunaScript® RT SuperMix Kit"},{"SortOrder":2,"Sku":"E3025","ProductName":"LunaScript® RT Master Mix Kit (Primer-free)"},{"SortOrder":3,"Sku":"E6560","ProductName":"ProtoScript® II First Strand cDNA Synthesis Kit"},{"SortOrder":4,"Sku":"E6300","ProductName":"ProtoScript® First Strand cDNA Synthesis Kit"},{"SortOrder":5,"Sku":"M0466","ProductName":"Template Switching RT Enzyme Mix"},{"SortOrder":6,"Sku":"M0681","ProductName":"Induro® Reverse Transcriptase"},{"SortOrder":7,"Sku":"M0368","ProductName":"ProtoScript® II Reverse Transcriptase"},{"SortOrder":8,"Sku":"M0253","ProductName":"M-MuLV Reverse Transcriptase"},{"SortOrder":9,"Sku":"M0277","ProductName":"AMV Reverse Transcriptase"},{"SortOrder":10,"Sku":"M0380","ProductName":"WarmStart® RTx Reverse Transcriptase"},{"SortOrder":11,"Sku":"M0439","ProductName":"WarmStart® RTx Reverse Transcriptase (Glycerol-free)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":12,"Category":"PCR Cleanup","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"T1130","ProductName":"Monarch® Spin PCR & DNA Cleanup Kit (5 μg)"},{"SortOrder":2,"Sku":"E1050","ProductName":"Exo-CIP™ Rapid PCR Cleanup Kit"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""}],"Products":{"Products":[{"SortOrder":1,"Sku":"E1555","ProductName":"LunaScript® Multiplex One-Step RT-PCR Kit"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":3,"Category":"DNA Modifying Enzymes & Cloning Technologies","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"Cloning & Synthetic Biology","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"NEBuilder® HiFi DNA Assembly Master Mix & Cloning Kit","ProductFamily":"1","ProductFamilyDescription":"Description: NEBuilder HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15–80 bp). It has utility for the synthetic biology community, as well as those interested in one-step cloning of multiple fragments due to its ease of use, flexibility and simple master-mix format. The reaction features different enzymes that perform in the same buffer. The end result is a double-stranded, fully sealed DNA molecule that can serve as template for PCR, RCA or a variety of other molecular biology applications, including direct transformation of E. coli.
\n\nNEBuilder HiFi Kits can be purchased with NEB 5-alpha Competent E. coli (Cloning Kit, NEB #E5520) or as a bundle with NEB 10-beta Competent E. coli (Bundle for Large Fragments, NEB #E2623). NEB 5-alpha competent cells are excellent for routine assemblies of 15 kb or less. NEB recommends NEB 10-beta competent cells for assemblies larger than 15 kb.\n
\nThe NEBuilder HiFi DNA Assembly Master Mix Includes:
\nThe NEBuilder HiFi DNA Assembly Cloning Kit Includes:
\nThe NEBuilder HiFi DNA Assembly Bundle for Large Fragments Includes:\n
\nDescription: Gibson Assembly Master Mix allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility.\n
\nGibson Assembly efficiently joins multiple overlapping DNA fragments in a single-tube isothermal reaction. The Gibson Assembly Master Mix includes three different enzymatic activities that perform in a single buffer:
\nThe Gibson Assembly Cloning Kit has been optimized for the assembly and cloning of up to 6 fragments.\n
\nThe Gibson Assembly Master Mix Includes:
\nThe Gibson Assembly Cloning Kit Includes:
\nDescription: The NEBridge Golden Gate Assembly Kits (BsaI-HFv2 and BsmBI-v2) contain an optimized mix of Type IIS restriction enzyme and T4 DNA Ligase. Together these enzymes can direct the assembly of multiple inserts using the Golden Gate method. The kits include pGGAselect destination plasmid, which provides a backbone for the assembly, features convenient restriction enzyme sites for subcloning, and has T7/SP6 promoter sequences to enable in vitro transcription.
\nNEBridge Ligase Master Mix performs high efficiency and high-fidelity Golden Gate Assemblies with a broad assortment of Type IIS restriction enzymes which can be ordered separately.
\nGolden Gate Assembly is a method for efficient and seamless assembly of DNA fragments using Type IIS restriction enzymes and T4 DNA Ligase. Type IIS restriction enzymes bind to their recognition sites but cut the DNA downstream from that site at a positional, not sequence-specific, cut site. Thus, a single Type IIS restriction enzyme can generate DNA fragments with unique overhangs (see Figure below). Ordered assembly of digested fragments proceeds through annealing of complementary overhangs on adjacent fragments. The final assembly product no longer contain Type IIS restriction enzyme recognition sites, so no further digestion is possible, allowing the assembly product to accumulate over time.
\nWhile particularly useful for multi-fragment assemblies, the Golden Gate method can also be used for cloning single inserts and inserts from diverse populations to create libraries. Golden Gate is also useful for assembling repetitive elements (e.g., gene circuits and CRISPR guide arrays).
\nAdvances in Ligase Fidelity: Research at NEB has led to increased understanding of ligase fidelity, including the development of a comprehensive method for profiling end-joining ligation fidelity in order to predict which overhangs have improved fidelity. This research allows careful choice of overhang sets, which is especially important for achieving complex Golden Gate Assemblies. To learn more, visit www.neb.com/goldengate.\n
\nNEBridge Golden Gate Assembly Kit (BsaI-HFv2) Includes:
\nNEBridge Golden Gate Assembly Kit (BsmBI-v2) Includes:
\nNEBridge Ligase Master Mix Includes:
\n* Note: Assemblies up to 24 fragments have been routinely achieved with both precloned and amplicon insert test systems. Assemblies of 35+ fragments have only used amplicon inserts to date.
","ProductFamilyIconCharacters":["r"],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E1601","ProductName":"NEBridge® Golden Gate Assembly Kit (BsaI-HF® v2)"},{"SortOrder":2,"Sku":"E1602","ProductName":"NEBridge® Golden Gate Assembly Kit (BsmBI-v2)"},{"SortOrder":3,"Sku":"M1100","ProductName":"NEBridge® Ligase Master Mix"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":4,"Category":"NEB® PCR Cloning Kit (with or without competent cells)","ProductFamily":"1","ProductFamilyDescription":"Description: The NEB PCR Cloning Kit contains optimized Cloning Master Mixes with a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background. It allows simple and quick cloning of any PCR amplicon, whether the amplification reactions are performed with proofreading DNA polymerases, such as Q5® or Phusion® which produce blunt ends; or nonproofreading DNA polymerases, such as Taq or Taq mixes (OneTaq, LongAmp Taq) which produce single-base overhangs. This is possible due to “invisible” end polishing components in the master mix that are active during the ligation step. The kit also allows direct cloning from amplification reactions without purification, and works well whether or not the primers used in the PCR possess 5´-phosphate groups.\n
\nKit Includes:
\nPhusion® is a registered trademark of Thermo Fisher Scientific
","ProductFamilyIconCharacters":["r"],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E1202","ProductName":"NEB® PCR Cloning Kit"},{"SortOrder":2,"Sku":"E1203","ProductName":"NEB® PCR Cloning Kit (Without Competent Cells)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":5,"Category":"Q5® Site-Directed Mutagenesis Kit (with or without competent cells)","ProductFamily":"1","ProductFamilyDescription":"Description: The Q5 Site-Directed Mutagenesis Kit allows rapid site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create substitutions, deletions and insertions in a wide variety of plasmids. Transformation into high-efficiency NEB 5-alpha Competent E. coli, provided with (NEB #E0554), ensures robust results with plasmids up to 14 kb in length.\n
\nApplications:
\nQ5 Site-Directed Mutagenesis Kit Includes:\n
\nKLD Enzyme Mix Includes:
\nDescription: T4 Polynucleotide Kinase catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5´ hydroxyl terminus of polynucleotides (double- and single-stranded DNA and RNA), as well as nucleoside 3´ monophosphates. The T4 Polynucleotide Kinase (NEB #M0201) also catalyzes the removal of 3´ phosphoryl groups from 3´ phosphoryl polynucleotides, deoxynucleoside 3´ monophosphates and deoxynucleoside 3´ diphosphates. The modified version (NEB #M0236) exhibits full kinase activity with no 3´ phosphatase activity.\n
\nReaction Conditions: 1X T4 Polynucleotide Kinase Reaction Buffer. Incubate at 37°C. Heat inactivation: 65°C for 20 minutes.\n
\nNotes: Fresh buffer is required for optimal activity (in older buffers, loss of DTT due to oxidation lowers activity).\n
\nCTP, GTP, TTP, UTP, dATP or dTTP can be substituted for ATP as a phosphate donor.\n
\nProtocols for phosphorylation (radioactive and non-radioactive) of DNA & RNA can be found at www.neb.com.\n
\nThe efficiencies of blunt and recessed 5´ end phosphorylation can be improved by heating to 70°C for 5 minutes, then chilling on ice prior to kinase addition and by adding PEG-8,000 to 5% (w/v).\n
\nT4 Polynucleotide Kinase requires ATP for activity, but the supplied reaction buffer does not contain ATP to allow for high specific activity radiolabeling reactions.\n
\nOften, a kinase reaction is followed by a ligation reaction. In such cases, the T4 PNK reaction is performed in ligase buffer at 37°C for 30 minutes. The product of this reaction can be used directly in the ligation reaction without a buffer change or heat inactivation UNLESS there is a need to keep other DNA fragments dephosphorylated during ligation. When this is desirable, PNK should be heat inactivated prior to ligation.\n
\nUnit Definition: One Richardson unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of acid-insoluble [32P] in 30 minutes at 37°C.
\nConcentration: 10,000 units/ml
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\nDNase I-XT is a salt tolerant enzyme that exhibits optimal activity between 50-100 mM salt and retains 65% and ~40% activity in 200 and 300 mM salt, respectively. Increased salt tolerance makes it ideal for DNA template removal from an in vitro transcription (IVT) reaction.
\nBoth enzymes are RNase-free, allowing for complete removal of DNA from RNA preps, while maintaining RNA integrity.
\nReaction Conditions: 1X DNase I or DNase I-XT Reaction Buffer, 37°C. DNase I can be heat inactivated at 75°C for 10 minutes, while DNase I-XT cannot.
\nReagents Supplied:
\nUnit Definition: DNase I – One unit is defined as the amount of enzyme which will completely degrade 1 µg of pBR322 DNA in a total reaction volume of 50 µl in 10 minutes at 37°C. DNase I-XT – One unit is defined as the amount of enzyme required to release 210 pmol of FAM from a 35-mer FAM-BHQ1 labeled hairpin oligonucleotide in 1 minute at 30°C in a 50 µl reaction volume.
\nConcentration: 2,000 units/ml
\nNotes: DNase I-XT is supplied with an optimized reaction buffer. Use with supplied buffer, and not DNase I Reaction Buffer. Likewise, due to the different salt concentration, do not use DNase I-XT Buffer with DNase I. When using DNase I, EDTA should be added to a final concentration of 5 mM to protect RNA from being degraded during enzyme inactivation.
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\nAfu Uracil-DNA Glycosylase (UDG) is a thermostable homolog of the E. coli Uracil-DNA Glycosylase (UDG) from Archaeoglobus fulgidus.
\nWarmStart Afu Uracil-DNA Glycosylase (UDG) from Archaeoglobus fulgidus is formulated with a reversibly-bound aptamer which inhibits its activity at temperatures below 42°C.
\nAntarctic Thermolabile UDG is sensitive to heat and can be rapidly and completely inactivated at temperatures above 50°C.
\nReaction Conditions: Uracil-DNA Glycosylase (UDG): UDG Reaction Buffer, 37°C. Afu Uracil-DNA Glycosylase (UDG) & WarmStart Afu Uracil-DNA Glycosylase (UDG): ThermoPol II (Mg-free) Reaction Buffer, 65°C. Antarctic Thermolabile UDG: Standard Taq Reaction Buffer, 37°C, heat inactivation: 50°C for 5 minutes.
\nUnit Definition:One unit is defined as the amount of enzyme required to release 60 pmol per minute of a fluorescently-labeled 47-mer single-stranded DNA oligonucleotide containing a single uracil base in 30 minutes. E. coli UDG is incubated at 37°C in a total reaction volume of 50 µl in 1X UDG Buffer. Afu UDG and WarmStart Afu UDG are incubated at 65°C in a total reaction volume of 50 µl in 1X Thermopol II Buffer. Antarctic Thermolabile UDG is incubated at at 30°C in a total reaction volume of 50 µl in 1X Standard Taq Reaction Buffer.
\nConcentration: E. coli UDG: 5,000 units/ml. Afu UDG, WarmStart Afu UDG: 2,000 units/ml. Antarctic Thermolabile UDG: 1,000 units/ml.
\nNote: UDG is active over a broad pH range, with an optimum pH 8.0, does not require divalent cation and is inhibited by high ionic strength (>200 mM). Afu UDG retains 50% activity in the presence of 150 mM NaCl. Afu UDG retains less than 1% activity after boiling for 30 minutes in standard reaction conditions. Under standard reaction conditions, uracil glycosylase inhibitor (UGI) does not inhibit Afu UDG.
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Description: USER (Uracil-Specific Excision Reagent) Enzyme generates a single nucleotide gap at the location of a uracil. USER Enzyme is a mixture of Uracil DNA glycosylase (UDG) and the DNA glycosylase-lyase Endonuclease VIII. UDG catalyses the excision of a uracil base, forming an abasic (apyrimidinic) site while leaving the phosphodiester backbone intact. The lyase activity of Endonuclease VIII breaks the phosphodiester backbone at the 3´ and 5´ sides of the abasic site so that base-free deoxyribose is released.
\nThermolabile Uracil DNA Glycosylase (UDG) catalyzes the excision of a uracil base, forming an abasic (apyrimidinic) site while leaving the phosphodiester backbone intact. The lyase activity of Endonuclease III breaks the phosphodiester backbone at the 3´ and 5´ sides of the abasic site. In addition to generating a different 3´- terminus than USER Enzyme, Thermolabile USER II Enzyme can also be completely heat inactivated after 10 minutes at 65°C.
\nThermostable USER III generates a single nucleotide gap at the location of a uracil. It is a mixture of Afu UDG and DNA glycosylase-lyase Endonuclease IV and is active between 50-75°C, with optimal activity observed at 65°C.
\nReaction Conditions: USER & Thermolabile USER II: rCutSmart Reaction Buffer, 37°C. Thermolabile USER II Enzyme can be heat inactivated at 65°C for 10 minutes, while USER cannot. Thermostable USER III: ThermoPol Reaction Buffer, 65°C.
\nUnit Definition: One unit is defined as the amount of enzyme required to nick 10 pmol of a 34-mer (USER & USER II) or 60-mer (USER III) oligonucleotide duplex containing a single uracil base, in 15 minutes at 37°C in a total reaction volume of 10 µl. Unit assay conditions can be found at www.neb.com.
\nConcentration: 1,000 units/ml
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\nP207–4 + H20 → 2HP04–2
\nSource: Pyrophosphatase, Inorganic (E. coli) is prepared from a clone of the E. coli inorganic pyrophosphatase gene.
\nPyrophosphatase, Inorganic (yeast) is an E. coli strain containing a genetic fusion of the Saccharomyces cerevisiae ppa gene and the gene coding for Mycobacterium xenopi GyrA intein. Developed by BioHelix Corporation, now a wholly owned subsidiary of Quidel Corporation, and produced at New England Biolabs.
\nThermostable Inorganic Pyrophosphatase is an E. coli strain carrying a plasmid encoding a pyrophosphatase from the extreme thermophile Thermococcus litoralis.
\nNudC is a NUDIX pyrophosphatase that efficiently hydrolyzes NAD+- and NADH-capped RNA, generating a ligatible 5´ monophosphate on the RNA (NAD+ decapping or deNADding). Deletion of the nudC gene has been shown to increase the fraction of NAD+- capped RNA in E. coli.
\nUnit Definition: One unit is defined as the amount\nof enzyme that will generate 1 µmol of phosphate per\nminute from inorganic pyrophosphate under standard\nreaction conditions.
\n1 µM of NudC hydrolyzes 200 µM or more NAD+\ninto NMN+ and AMP in 1X NEBuffer r3.1 and 5 mM DTT at\n37°C for 30 min.
\nConcentration: Pyrophosphatase, Inorganic\n(E. coli) and Pyrophosphatase, Inorganic (yeast)\n100 units/ml. Thermostable Inorganic Pyrophosphatase\n2,000 units/ml. NudC Pyrophosphatase: 10 µM
\nNudC Pyrophosphatase is an Enzyme for Innovation\n(EFI). EFI is a project initiated by NEB to provide unique\nenzymes to the scientific community in the hopes of\nenabling the discovery of new and innovative applications. Visit www.neb.com/EnzymesforInnovation\nto view the full list.
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DNA"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":13,"Category":"Other","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"N0315","ProductName":"M13KO7 Helper Phage"},{"SortOrder":2,"Sku":"M0665","ProductName":"Tth Argonaute (TtAgo)"},{"SortOrder":3,"Sku":"M0669","ProductName":"EnGen® SpRY Cas9"},{"SortOrder":4,"Sku":"T1041","ProductName":"Cryogenic Cold Storage Box"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":14,"Category":"Competent Cells for Cloning","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"C2987","ProductName":"NEB® 5-alpha Competent E. coli (High Efficiency)"},{"SortOrder":2,"Sku":"C2984","ProductName":"NEB® Turbo Competent E. coli (High 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DNA and RNA purified with Monarch kits is highly pure and suitable for use in a wide range of applications, including IVT RNA synthesis, sequencing, cloning, PCR and other enzymatic manipulations. Monarch kits are developed for performance and with sustainability in mind; they use significantly less plastic and are packaged in responsibly-sourced, recyclable material. For flexibility, select Monarch kit components are available separately. Learn more at NEBmonarch.com.","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"T1110","ProductName":"Monarch® Spin Plasmid Miniprep Kit"},{"SortOrder":2,"Sku":"T1120","ProductName":"Monarch® Spin DNA Gel Extraction Kit"},{"SortOrder":3,"Sku":"T1130","ProductName":"Monarch® Spin PCR & DNA Cleanup Kit (5 μg)"},{"SortOrder":4,"Sku":"T3010","ProductName":"Monarch® Spin gDNA Extraction Kit"},{"SortOrder":5,"Sku":"T3060","ProductName":"Monarch® HMW DNA Extraction Kit for Tissue"},{"SortOrder":6,"Sku":"T3050","ProductName":"Monarch® HMW DNA Extraction Kit for Cells & Blood"},{"SortOrder":7,"Sku":"T2030","ProductName":"Monarch® Spin RNA Cleanup Kit (10 μg)"},{"SortOrder":8,"Sku":"T2040","ProductName":"Monarch® Spin RNA Cleanup Kit (50 µg)"},{"SortOrder":9,"Sku":"T2050","ProductName":"Monarch® Spin RNA Cleanup Kit (500 µg)"},{"SortOrder":10,"Sku":"T1117","ProductName":"Monarch® Spin Columns S2D and Tubes"},{"SortOrder":11,"Sku":"T2047","ProductName":"Monarch® Spin Columns S2A"},{"SortOrder":12,"Sku":"T3017","ProductName":"Monarch® Spin Columns S2C and Tubes"},{"SortOrder":13,"Sku":"T2037","ProductName":"Monarch® Spin Columns S1A and Tubes"},{"SortOrder":14,"Sku":"T2057","ProductName":"Monarch® Spin Columns S2B and Tubes"},{"SortOrder":15,"Sku":"T4010","ProductName":"Monarch® Mag Viral DNA/RNA Extraction Kit"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"1","DisplayAsTableColumns":[{"FieldName":"FullProductName","ColumnDisplayName":"Product"},{"FieldName":"CatalogNumber","ColumnDisplayName":"NEB #"},{"FieldName":"Size","ColumnDisplayName":"Size"}],"GroupFieldName":""}],"Products":{"Products":[]},"ImageMetadata":[],"Charts":[{"ChartKind":"Static","ChartTitle":"Properties of Exonucleases and Non-specific Endonucleases","ChartCode":"Properties of Exonucleases and Nonspecific Endonucleases"}],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":4,"Category":"Nucleic Acid Purification","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"Plasmid Purification","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"T1110","ProductName":"Monarch® Spin Plasmid Miniprep Kit"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":2,"Category":"DNA Cleanup","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"T1130","ProductName":"Monarch® Spin PCR & DNA Cleanup Kit (5 μg)"},{"SortOrder":2,"Sku":"T1120","ProductName":"Monarch® Spin DNA Gel Extraction Kit"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":3,"Category":"Genomic DNA Purification","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"T3010","ProductName":"Monarch® Spin gDNA Extraction Kit"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":4,"Category":"HMW DNA Extraction","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"Monarch® HMW DNA Extraction Kits","ProductFamily":"1","ProductFamilyDescription":"Description: Monarch HMW DNA Extraction Kits provide a rapid and reliable process for extracting high molecular weight DNA (HMW DNA) from biological samples including cells, blood, tissue, bacteria and other sample types. Utilizing an optimized process that combines gentle cell lysis with a tunable fragment length generation, followed by precipitation of the extracted DNA onto the surface of large glass beads, the prep proceeds rapidly. DNA size ranges from 50-250 kb for the standard protocol and into the Mb range on several sample types when the lowest agitation speeds are used. Purified DNA is recovered in high yield with excellent purity, including nearly complete removal of RNA. Purified HMW gDNA is easy to dissolve and is suitable for a variety of downstream applications including long-read sequencing (e.g., PacBio® and Oxford Nanopore Technologies®).
\nKits Include:
\nDescription: The Monarch Spin RNA Cleanup Kits provide a fast and simple silica spin column-based solution for RNA cleanup and concentration after any enzymatic reaction (including in vitro transcription, DNase I treatment, capping and labeling) and after other purification methods such as phenol/chloroform extraction. The Monarch Spin RNA Cleanup Kits are available in 3 different binding capacities: 10 μg, 50 μg and 500 μg. Each kit contains unique columns, all designed to prevent buffer retention and ensure no carryover of contaminants, enabling low-volume elution of highly-pure RNA. Following the standard protocol, RNA ≥ 25 nt is purified with this kit; however, a modified protocol is available to enable the binding of RNA as small as 15 nt (including miRNAs).
\nKits Include:\n
\nDescription: NEBNext Ultra II DNA Library Prep Kits for Illumina meet the challenge of constructing high quality libraries from ever-decreasing input quantities, enabling high yield preparation of high quality libraries from 100 picograms to 1 microgram of input DNA. Ultra II kits use a fast, streamlined, automatable workflow and enable use of fewer PCR cycles while also improving GC coverage. The kit is also effective with challenging samples such as FFPE DNA.
\nThe Ultra II FS DNA Library Prep Kit combines robust enzymatic DNA fragmentation with end repair and dA-tailing, integrated into a streamlined library prep workflow.
\nPCR-free kits are now available for both the Ultra II DNA and Ultra II FS DNA workflows.
\nThe Ultra II FS kits are available with or without SPRIselect® beads.
\nDescription: NEBNext Enzymatic Methyl-seq (EM-seq™) is a high-performance enzyme-based alternative to bisulfite conversion for the identification of 5mC and 5hmC. Unlike bisulfite conversion, this highly efficient method minimizes DNA damage, resulting in superior detection of methylated cytosines, with fewer sequencing reads.
\nThe new NEBNext Enzymatic Methyl-seq v2 Kit has a wider input range (as low as 100 pg) and a faster, more streamlined workflow than the original EM-seq kit (NEB #E7120).
\nThe NEBNext Enzymatic Methyl-seq v2 Kit includes conversion reagents, library prep reagents and the EM-seq Adaptor. Multiple sets of the required index primers (NEBNext LV Unique Dual Index Primers) are available separately, enabling greater flexibility in multiplexing.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"e8015","ProductName":"NEBNext® Enzymatic Methyl-seq v2 Kit"},{"SortOrder":2,"Sku":"E8020","ProductName":"NEBNext® Enzymatic Methyl-seq v2 Conversion Module"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":2,"Category":"NEBNext® Enzymatic Methyl-seq (EM-seq™)","ProductFamily":"1","ProductFamilyDescription":"Description: While bisulfite sequencing has been the gold standard for the study of DNA methylation, this conversion treatment is damaging to DNA, resulting in DNA fragmentation, loss and GC bias. The NEBNext Enzymatic Methyl-seq Kit (EM-seq™) provides an enzymatic alternative to whole genome bisulfite sequencing (WGBS), combined with highefficiency streamlined library preparation suitable for Illumina sequencing.
\nThe highly effective EM-seq enzymatic conversion minimizes damage to DNA and, in combination with the supplied NEBNext Ultra II library preparation workflow reagents, results in high quality libraries that enable superior detection of 5mC and 5hmC from fewer sequencing reads.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E7120","ProductName":"NEBNext® Enzymatic Methyl-seq Kit"},{"SortOrder":2,"Sku":"E7125","ProductName":"NEBNext® Enzymatic Methyl-seq Conversion Module"},{"SortOrder":3,"Sku":"M0597","ProductName":"NEBNext® Q5U® Master Mix"},{"SortOrder":4,"Sku":"E7140","ProductName":"NEBNext® Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":3,"Category":"NEBNext® Enzymatic 5hmC-seq (E5hmC-seq™)","ProductFamily":"1","ProductFamilyDescription":"Description: While NEBNext Enzymatic Methyl-seq (EM-seq) detects both 5mC and 5hmC, it does not distinguish between them. Specific detection of 5hmC sites is now enabled by the NEBNext Enzymatic 5hmC-seq Kit (E5hmC-seq™). The kit includes NEBNext Ultra II library prep reagents, and 5hmC is detected using a two-step enzymatic conversion workflow (Figure 1), that minimizes damage to DNA and allows discrimination of 5hmC from both cytosine and 5mC after Illumina sequencing. E5hmC-seq data can also be subtracted from EM-seq data, allowing determination of the precise location of individual 5mC and 5hmC sites.","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E3350","ProductName":"NEBNext® Enzymatic 5hmC-seq Kit"},{"SortOrder":2,"Sku":"E3365","ProductName":"NEBNext® Enzymatic 5hmC-seq Conversion Module"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""}],"Products":{"Products":[]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":6,"Category":"Reagents for RNA Depletion","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"NEBNext® rRNA Depletion Kits (Human/Mouse/Rat and Bacteria)","ProductFamily":"1","ProductFamilyDescription":"Description: The NEBNext rRNA Depletion Kit (Human/ Mouse/Rat) employs an RNase H-based method (1,2) to deplete cytoplasmic (5S, 5.8S, 18S, 28S, human ITS, ETS) and mitochondrial (12S and 16S) rRNA from human total RNA preparations.
\nSpecific enrichment of bacterial mRNAs is challenging due to their lack of poly(A) tails, precluding the use of oligo d(T)-based enrichment methods. For these samples, specific removal of bacterial rRNAs is an\nefficient way to enrich for RNAs of interest.\n
\nThe NEBNext rRNA Depletion Kit (Bacteria) targets\nremoval of rRNA (5S, 16S and 23S) from gram-positive\nand gram-negative organisms, from monocultures or\nsamples with mixed bacterial species.
\nDescription: The great majority of RNA in blood samples is comprised of globin mRNA as well as cytoplasmic and mitochondrial ribosomal RNAs (rRNA). These highly abundant RNA species can conceal the biological significance of less abundant transcripts, and so their efficient and specific removal is desirable.
\nThe NEBNext Globin & rRNA Depletion Kit (Human/ Mouse/Rat) employs the NEBNext RNase H-based RNA depletion workflow to deplete the following:
\nThe kit is effective with human, mouse and rat total RNA\npreparations, both intact and degraded. The resulting\ndepleted RNA is suitable for RNA-seq, random-primed\ncDNA synthesis, or other downstream RNA analysis.\n
\nThis kit can also be used following poly(A) mRNA\nenrichment (e.g., using the NEBNext poly(A) mRNA\nMagnetic Isolation Module, NEB #E7490), so that the\nfinal depleted RNA contains only mRNA of interest and\nno non-coding RNA.\n
\nThis kit is available with or without RNAClean beads.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E7750","ProductName":"NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat)"},{"SortOrder":2,"Sku":"E7755","ProductName":"NEBNext® Globin & rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""}],"Products":{"Products":[{"SortOrder":1,"Sku":"E7865","ProductName":"NEBNext® RNA Depletion Core Reagent Set"},{"SortOrder":2,"Sku":"E7870","ProductName":"NEBNext® RNA Depletion Core Reagent Set with RNA Sample Purification Beads"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":7,"Category":"Customized RNA Depletion","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"Customized Depletion of Unwanted RNA","ProductFamily":"1","ProductFamilyDescription":"Description: In RNA-seq, highly expressed transcripts\nwith minimal biological interest, such as ribosomal\nRNA (rRNA) can dominate readouts and mask detection\nof more informative low-abundance transcripts. This\nchallenge is amplified when working with sample types\nfor which pre-designed RNA depletion kits are not\navailable. The NEBNext RNA Depletion Core Reagent set\nprovides a customized approach to deplete unwanted\nRNA from any organism, using probe sequences\ndesigned with the user-friendly NEBNext Custom RNA\nDepletion Design Tool.\n
\nThe efficient RNase-H-based workflow, and close tiling of\nprobes designed using the online tool, enables effective\ndepletion from both low- and high-quality RNA, with a broad range of input amounts.\n
\nSTEP 1: Use the online NEBNext Custom RNA\nDepletion Design Tool to obtain custom probe\nsequences, by entering the sequence of your\ntarget RNA.\n
\nSTEP 2: Order ssDNA probe oligonucleotides from\nyour trusted oligo provider.\n
\nSTEP 3: Use the probes with the NEBNext Custom RNA\nDepletion Core Reagent Set or in combination\nwith other NEBNext RNA Depletion Kits
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E7865","ProductName":"NEBNext® RNA Depletion Core Reagent Set"},{"SortOrder":2,"Sku":"E7870","ProductName":"NEBNext® RNA Depletion Core Reagent Set with RNA Sample Purification Beads"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""}],"Products":{"Products":[]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":8,"Category":"mRNA Isolation","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E3370","ProductName":"NEBNext® High Input Poly(A) mRNA Isolation Module"},{"SortOrder":2,"Sku":"E7490","ProductName":"NEBNext® Poly(A) mRNA Magnetic Isolation Module"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":9,"Category":"Single Cell RNA","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"NEBNext® Single Cell/Low Input RNA Library Prep","ProductFamily":"1","ProductFamilyDescription":"Description: The unique workflow of the NEBNext\nSingle Cell/Low Input RNA Library Prep Kit for Illumina\nmeets the demand for a highly sensitive, yet robust\nmethod that consistently generates high-quality, fulllength transcript sequencing data from a single cell or\nultra-low input RNA.\n
\nOptimized cDNA synthesis and amplification steps\nincorporate template switching, and utilize a unique\nprotocol and suite of reagents.
\ncDNAs are generated directly from single cells or\n2 pg–200 ng RNA, and even low-abundance transcripts\nare represented in the high yields of cDNA obtained.\nThis is followed by library construction that incorporates\nthe Ultra II FS enzymatic DNA fragmentation/end repair/\ndA-tailing mix in a simple and efficient workflow.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E6420","ProductName":"NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina®"},{"SortOrder":2,"Sku":"E6421","ProductName":"NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module"},{"SortOrder":3,"Sku":"E5530","ProductName":"NEBNext® Single Cell Lysis Module"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""}],"Products":{"Products":[]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":10,"Category":"Small RNA","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"NEBNext® Small RNA Library Prep Kits","ProductFamily":"1","ProductFamilyDescription":"Description: The novel NEBNext Small RNA workflow has been optimized to minimize adaptor-dimers while producing high-yield, high-diversity libraries. Adaptors and primers are included in the Small RNA kits, and multiplexing options are available. The Multiplex kit contains index primers, and the Multiplex-Compatible kit enables use with your own barcode primers.","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E7300","ProductName":"NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)"},{"SortOrder":2,"Sku":"E7580","ProductName":"NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)"},{"SortOrder":3,"Sku":"E7560","ProductName":"NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)"},{"SortOrder":4,"Sku":"E7330","ProductName":"NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""}],"Products":{"Products":[]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":11,"Category":"Magnetic Separation","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"Magnetic Separation","ProductFamily":"1","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"S1515","ProductName":"NEBNext® Magnetic Separation Rack"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"1","DisplayAsTableColumns":[{"FieldName":"FullProductName","ColumnDisplayName":"Product"},{"FieldName":"CatalogNumber","ColumnDisplayName":"NEB #"},{"FieldName":"Size","ColumnDisplayName":"Size"}],"GroupFieldName":""}],"Products":{"Products":[{"SortOrder":1,"Sku":"S1515","ProductName":"NEBNext® Magnetic Separation Rack"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":12,"Category":"Modules and Enzymes","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"Modules & Enzymes for DNA Library Prep","ProductFamily":"1","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E8020","ProductName":"NEBNext® Enzymatic Methyl-seq v2 Conversion Module"},{"SortOrder":2,"Sku":"E3365","ProductName":"NEBNext® Enzymatic 5hmC-seq Conversion Module"},{"SortOrder":3,"Sku":"E7360","ProductName":"NEBNext FFPE DNA Repair v2 Module"},{"SortOrder":4,"Sku":"M6630","ProductName":"NEBNext® FFPE DNA Repair Mix"},{"SortOrder":5,"Sku":"E2612","ProductName":"NEBNext® Microbiome DNA Enrichment Kit"},{"SortOrder":6,"Sku":"M7634","ProductName":"NEBNext UltraShear®Adaptors and Primers for RNA Library Prep
","ProductFamily":"1","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E7416","ProductName":"NEBNext® Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA Set 1)"},{"SortOrder":2,"Sku":"E6440","ProductName":"NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs)"},{"SortOrder":3,"Sku":"E6442","ProductName":"NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 2)"},{"SortOrder":4,"Sku":"E6444","ProductName":"NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 3)"},{"SortOrder":5,"Sku":"E6446","ProductName":"NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 4)"},{"SortOrder":6,"Sku":"E6448","ProductName":"NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 5)"},{"SortOrder":7,"Sku":"E7600","ProductName":"NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1) "},{"SortOrder":8,"Sku":"E7780","ProductName":"NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 2)"},{"SortOrder":9,"Sku":"E7335","ProductName":"NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)"},{"SortOrder":10,"Sku":"E7500","ProductName":"NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)"},{"SortOrder":11,"Sku":"E7710","ProductName":"NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)"},{"SortOrder":12,"Sku":"E7730","ProductName":"NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)"},{"SortOrder":13,"Sku":"E6609","ProductName":"NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)"},{"SortOrder":14,"Sku":"B1430","ProductName":"NEBNext® Adaptor Dilution Buffer"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"1","DisplayAsTableColumns":[{"FieldName":"FullProductName","ColumnDisplayName":"Product"},{"FieldName":"CatalogNumber","ColumnDisplayName":"NEB #"},{"FieldName":"Size","ColumnDisplayName":"Size"}],"GroupFieldName":""},{"SortOrder":3,"Category":"NEBNext Adaptors & Primers for Illumina","ProductFamily":"1","ProductFamilyDescription":"Description: Designed for use in library prep for DNA, ChIP DNA and RNA (but not Small RNA), the NEBNext Multiplex Oligos for Illumina are an essential component of the NGS sample prep workflow. Offering a range of indexing strategies, from Unique Dual Index UMI Adaptors to the truncated, hairpin-loop NEBNext Adaptor, meant for use with UDI, dual, and single index primers, the NEBNext Multiplex Oligos can support NGS across a wide range of formats. Optimized for performance in recommended applications, there’s an NEBNext indexing option tailored to you. NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.Description: The NEBNext ARTIC kits were developed in response to the critical need for reliable and accurate methods for sequencing viral pathogens, specifically SARS-CoV-2. These kits, for long and short read sequencing, were based on the original work of the ARTIC Network (1). The ARTIC SARS-CoV-2 sequencing workflow is a multiplexed amplicon-based whole-viral-genome sequencing approach.
\nNEBNext ARTIC Companion kits include primers and reagents for RT-PCR from SARS-CoV-2 gRNA and downstream library preparation for Illumina and Oxford Nanopore Technologies sequencing.
\nThe optimized primers and reagents for RT-PCR deliver uniform, ample amplicon yields from gRNA across a wide copy number range, and library prep and sequencing can be performed downstream of a single RT-PCR procedure.
\nFor Illumina applications, a novel DNA polymerase formulation for the enrichment of next-generation sequencing libraries eliminates the need to normalize amplicon concentrations prior to library preparation. The NEBNext ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina) incorporates enzymatic cDNA fragmentation, and generates libraries with inserts in the 150 bp range.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E7658","ProductName":"NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®)"},{"SortOrder":2,"Sku":"E7660","ProductName":"NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®)"},{"SortOrder":3,"Sku":"E7626","ProductName":"NEBNext® ARTIC SARS-CoV-2 RT-PCR Module"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":15,"Category":"Reagents for Oxford Nanopore Technologies Sequencing","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"Products for Oxford Nanopore DNA Library Prep","ProductFamily":"1","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E7180","ProductName":"NEBNext® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing"},{"SortOrder":2,"Sku":"E7672","ProductName":"NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing"},{"SortOrder":3,"Sku":"E7660","ProductName":"NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"1","DisplayAsTableColumns":[{"FieldName":"FullProductName","ColumnDisplayName":"Product"},{"FieldName":"CatalogNumber","ColumnDisplayName":"NEB #"},{"FieldName":"Size","ColumnDisplayName":"Size"}],"GroupFieldName":""},{"SortOrder":2,"Category":"Products for Oxford Nanopore RNA Library Prep","ProductFamily":"1","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E7660","ProductName":"NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"1","DisplayAsTableColumns":[{"FieldName":"FullProductName","ColumnDisplayName":"Product"},{"FieldName":"CatalogNumber","ColumnDisplayName":"NEB #"},{"FieldName":"Size","ColumnDisplayName":"Size"}],"GroupFieldName":""},{"SortOrder":3,"Category":"Reagents for Oxford Nanopore Technologies® Sequencing","ProductFamily":"1","ProductFamilyDescription":"Description: Many NEBNext and NEB products are recommended for use in multiple sample prep workflows for Oxford Nanopore Technologies sequencing, for a range of sample types and applications.
\nThe NEBNext Companion Module v2 for Oxford Nanopore Technologies Ligation Sequencing includes the NEBNext DNA repair, end repair and ligations reagents recommended for Oxford Nanopore Technologies updated SQK-LSK114 singleplex ligation sequencing library prep protocols.
\nDescription: The NEBNext Immune Sequencing Kits (Human & Mouse) enable exhaustive profiling of somatic mutations in the full-length immune gene repertoires of B cells and T cells, via the expression of complete antibody chains. A unique, UMI-based mRNA barcoding process allows PCR copies derived from an individual molecule to be converted to a consensus sequence. This improves sequence accuracy and eliminates PCR bias.
\nImmune repertoire sequencing is frequently used to\nanalyze immune responses, both current and distant.\nAreas of particular interest include characterization\nof autoimmune diseases, oncology, discovery of\nneutralizing antibodies against infectious disease,\ntumor-infiltrating lymphocytes and use as a tool to study\nresidual disease. Recent improvements in read lengths\nand throughputs of next-generation sequencing (NGS)\nplatforms have resulted in a rise in the popularity of\nimmune repertoire sequencing.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E6320","ProductName":"NEBNext® Immune Sequencing Kit (Human)"},{"SortOrder":2,"Sku":"E6330","ProductName":"NEBNext® Immune Sequencing Kit (Mouse)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""}],"Products":{"Products":[{"SortOrder":1,"Sku":"E6320","ProductName":"NEBNext® Immune Sequencing Kit (Human)"},{"SortOrder":2,"Sku":"E6330","ProductName":"NEBNext® Immune Sequencing Kit (Mouse)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":18,"Category":"DNA Separation","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"DNA Enrichment","ProductFamily":"1","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E2612","ProductName":"NEBNext® Microbiome DNA Enrichment Kit"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"1","DisplayAsTableColumns":[{"FieldName":"FullProductName","ColumnDisplayName":"Product"},{"FieldName":"CatalogNumber","ColumnDisplayName":"NEB #"},{"FieldName":"Size","ColumnDisplayName":"Size"}],"GroupFieldName":""}],"Products":{"Products":[{"SortOrder":1,"Sku":"E2612","ProductName":"NEBNext® Microbiome DNA Enrichment Kit"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":19,"Category":"Library Quantitation","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"Library Quantitation DNA Library Prep","ProductFamily":"1","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E7630","ProductName":"NEBNext® Library Quant Kit for Illumina®"},{"SortOrder":2,"Sku":"E3410","ProductName":"NEBNext® Library Quant Kit for Ultima Genomics®"},{"SortOrder":3,"Sku":"B6118","ProductName":"NEBNext® Library Dilution Buffer"},{"SortOrder":4,"Sku":"E7642","ProductName":"NEBNext® Library Quant DNA Standards"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"1","DisplayAsTableColumns":[{"FieldName":"FullProductName","ColumnDisplayName":"Product"},{"FieldName":"CatalogNumber","ColumnDisplayName":"NEB #"},{"FieldName":"Size","ColumnDisplayName":"Size"}],"GroupFieldName":""},{"SortOrder":2,"Category":"Library Quantitation RNA Library Prep","ProductFamily":"1","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E7630","ProductName":"NEBNext® Library Quant Kit for Illumina®"},{"SortOrder":2,"Sku":"E3410","ProductName":"NEBNext® Library Quant Kit for Ultima Genomics®"},{"SortOrder":3,"Sku":"B6118","ProductName":"NEBNext® Library Dilution Buffer"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"1","DisplayAsTableColumns":[{"FieldName":"FullProductName","ColumnDisplayName":"Product"},{"FieldName":"CatalogNumber","ColumnDisplayName":"NEB #"},{"FieldName":"Size","ColumnDisplayName":"Size"}],"GroupFieldName":""},{"SortOrder":3,"Category":"NEBNext® Library Quant Kits","ProductFamily":"1","ProductFamilyDescription":"Accurate quantitation of next-generation sequencing (NGS) libraries is essential for optimizing data yield and quality from each sequencing run. Precise quantitation ensures that the appropriate amount of library is loaded onto the NGS platform, facilitating reliable predictions of sequencing output. Quantitative PCR (qPCR) is recognized as the most accurate and effective method for library quantitation, offering significantly higher consistency and reproducibility compared to alternative techniques. Amplification-based methods, such as qPCR, target adaptor-containing sequences, providing a more accurate estimate of the concentration of library molecules available for sequencing.
\nNEBNext library quant kits are optimized with platform-specific reagents to ensure efficient amplification of Illumina® or Ultima® genomic NGS libraries. The streamlined protocol minimizes pipetting steps and uses a single extension time for all libraries, enhancing efficiency while generating reproducible, high-quality results.
\nThe NEBNext Library Quant Kit for Illumina is designed for precise qPCR-based quantitation of NGS libraries prepared for Illumina sequencing platforms. This kit includes primers targeting the P5 and P7 Illumina adaptor sequences and pre-diluted standards for reliable quantitation of DNA libraries ranging from 150 to 1,000 bp. It offers flexibility with the option to use either four or six standards.
\nThe NEBNext® Library Quant Kit for Ultima Genomics® delivers precise and reliable qPCR-based quantitation of NGS libraries prepared for the Ultima Genomics UG 100™ sequencing platform. The kit features forward and reverse primers targeting the Ultima adaptor sequence, and includes five high-quality, pre-diluted DNA standards for reliable quantitation of diluted DNA libraries. Additionally, it incorporates the Luna® Universal qPCR Master Mix with a blue, non-interfering, visible tracking dye, which can also serve as a passive reference dye, eliminating the need for additional ROX in real-time instruments requiring ROX normalization.
\nNEBNext library quant kits ensure unparalleled accuracy and reliability in NGS library quantitation, giving users confidence in their results.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E7630","ProductName":"NEBNext® Library Quant Kit for Illumina®"},{"SortOrder":2,"Sku":"E3410","ProductName":"NEBNext® Library Quant Kit for Ultima Genomics®"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""}],"Products":{"Products":[{"SortOrder":1,"Sku":"E7630","ProductName":"NEBNext® Library Quant Kit for Illumina®"},{"SortOrder":2,"Sku":"B6118","ProductName":"NEBNext® Library Dilution Buffer"},{"SortOrder":3,"Sku":"E3410","ProductName":"NEBNext® Library Quant Kit for Ultima Genomics®"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":20,"Category":"Reagents for Ion Torrent","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"Products for Ion Torrent DNA Library Prep","ProductFamily":"1","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E6270","ProductName":"NEBNext® Fast DNA Library Prep Set for Ion Torrent®"},{"SortOrder":2,"Sku":"E6285","ProductName":"NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent®"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"1","DisplayAsTableColumns":[{"FieldName":"FullProductName","ColumnDisplayName":"Product"},{"FieldName":"CatalogNumber","ColumnDisplayName":"NEB #"},{"FieldName":"Size","ColumnDisplayName":"Size"}],"GroupFieldName":""},{"SortOrder":2,"Category":"Reagents for Ion Torrent","ProductFamily":"1","ProductFamilyDescription":"Description: NEBNext kits are available for DNA library preparation for Ion Torrent, with or without enzymatic DNA fragmentation. In addition to stringent QCs on individual components, the NEBNext DNA kits are functionally validated by library preparation of a genomic DNA library, followed by Ion Torrent sequencing. Reagent lots are reserved specifically for inclusion in NEBNext kits. Most of these reagents are provided in master mix format, reducing the number of vials provided in the kits, and reducing pipetting steps. Adaptors and primers for singleplex libraries are supplied in the kits. For multiplexed libraries, the Ion XPress™ Barcode Adaptors from Thermo Fisher Scientific can be used.","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E6285","ProductName":"NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent®"},{"SortOrder":2,"Sku":"E6270","ProductName":"NEBNext® Fast DNA Library Prep Set for Ion Torrent®"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""}],"Products":{"Products":[]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":21,"Category":"Reagents Suitable for Any Platform","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":22,"Category":"FFPE DNA Repair","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"DNA Repair","ProductFamily":"1","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E7360","ProductName":"NEBNext FFPE DNA Repair v2 Module"},{"SortOrder":2,"Sku":"M6630","ProductName":"NEBNext® FFPE DNA Repair Mix"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"1","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":2,"Category":"NEBNext® FFPE DNA Library Prep & UltraShear® FFPE DNA Library Prep Kits","ProductFamily":"1","ProductFamilyDescription":"Description: FFPE DNA poses many challenges for library preparation, including characteristically low input amounts and highly variable damage from fixation, storage, and extraction methods. Regions of interest are often enriched using hybrid capture-based approaches – these workflows require high inputs of diverse, uniform DNA libraries.
\nThe NEBNext FFPE DNA Library Prep Kit includes the NEBNext FFPE DNA Repair v2 Module, an optimized cocktail of enzymes designed to repair FFPE DNA, library prep reagents featuring a new polymerase master mix, and a protocol optimized for FFPE DNA. The NEBNext UltraShear FFPE DNA Library Prep Kit also includes NEBNext UltraShear, a new solution designed for enzymatic fragmentation of challenging samples (e.g., FFPE DNA). This enzymatic shearing solution further increases library yields and quality, while eliminating artifacts typical of other enzymatic fragmentation approaches.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E6650","ProductName":"NEBNext® FFPE DNA Library Prep Kit"},{"SortOrder":2,"Sku":"E6655","ProductName":"NEBNext UltraShear® FFPE DNA Library Prep KitNEB offers a variety of DNA Ladders with sizes ranging from\n10 bp to 48.5 kb for use in agarose gel electrophoresis.
\nOur 1 kb Plus, 1 kb and 100 bp DNA Ladders are offered in four formats.\nConventional ladders are supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS. Quick-Load ladders use either non-fluorescing, purple dye or bromophenol blue as a tracking dye. TriDye ladders contain three dyes to facilitate monitoring of gel migration. Note that the TriDye Ultra Low Range DNA Ladder is suitable for both native polyacrylamide and agarose gels.
","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"Quick-Load Purple Formats","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"N0550","ProductName":"Quick-Load® Purple 1 kb Plus DNA Ladder"},{"SortOrder":2,"Sku":"N0552","ProductName":"Quick-Load® Purple 1 kb DNA Ladder"},{"SortOrder":3,"Sku":"N0551","ProductName":"Quick-Load® Purple 100 bp DNA Ladder"},{"SortOrder":4,"Sku":"N0556","ProductName":"Quick-Load® Purple 50 bp DNA Ladder"},{"SortOrder":5,"Sku":"N0557","ProductName":"Quick-Load® Purple Low Molecular Weight DNA Ladder"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":2,"Category":"Other Ready-to-Load Formats","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"N0559","ProductName":"1 kb Plus DNA Ladder for Safe Stains"},{"SortOrder":2,"Sku":"N3238","ProductName":"Fast DNA Ladder"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":3,"Category":"TriDye Formats","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"N3270","ProductName":"TriDye™ 1 kb Plus DNA Ladder"},{"SortOrder":2,"Sku":"N3272","ProductName":"TriDye™ 1 kb DNA Ladder"},{"SortOrder":3,"Sku":"N3271","ProductName":"TriDye™ 100 bp DNA Ladder"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":4,"Category":"Quick-Load Formats","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"N0469","ProductName":"Quick-Load® 1 kb Plus DNA Ladder"},{"SortOrder":2,"Sku":"N0468","ProductName":"Quick-Load® 1 kb DNA Ladder"},{"SortOrder":3,"Sku":"N3239","ProductName":"Quick-Load® 1 kb Extend DNA Ladder"},{"SortOrder":4,"Sku":"N0467","ProductName":"Quick-Load® 100 bp DNA Ladder"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""}],"Products":{"Products":[{"SortOrder":1,"Sku":"N0550","ProductName":"Quick-Load® Purple 1 kb Plus DNA Ladder"},{"SortOrder":2,"Sku":"N0552","ProductName":"Quick-Load® Purple 1 kb DNA Ladder"},{"SortOrder":3,"Sku":"N0551","ProductName":"Quick-Load® Purple 100 bp DNA Ladder"},{"SortOrder":4,"Sku":"N0556","ProductName":"Quick-Load® Purple 50 bp DNA Ladder"},{"SortOrder":5,"Sku":"N0557","ProductName":"Quick-Load® Purple Low Molecular Weight DNA Ladder"},{"SortOrder":6,"Sku":"N0559","ProductName":"1 kb Plus DNA Ladder for Safe Stains"},{"SortOrder":7,"Sku":"N3238","ProductName":"Fast DNA Ladder"},{"SortOrder":8,"Sku":"N3270","ProductName":"TriDye™ 1 kb Plus DNA Ladder"},{"SortOrder":9,"Sku":"N3272","ProductName":"TriDye™ 1 kb DNA Ladder"},{"SortOrder":10,"Sku":"N3271","ProductName":"TriDye™ 100 bp DNA Ladder"},{"SortOrder":11,"Sku":"N0558","ProductName":"TriDye™ Ultra Low Range DNA Ladder"},{"SortOrder":12,"Sku":"N0469","ProductName":"Quick-Load® 1 kb Plus DNA Ladder"},{"SortOrder":13,"Sku":"N0468","ProductName":"Quick-Load® 1 kb DNA Ladder"},{"SortOrder":14,"Sku":"N3239","ProductName":"Quick-Load® 1 kb Extend DNA Ladder"},{"SortOrder":15,"Sku":"N0467","ProductName":"Quick-Load® 100 bp DNA Ladder"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":3,"Category":"PFG Ladders","ProductFamily":"1","ProductFamilyDescription":"The Lambda PFG Ladder consists of one GelSyringe dispenser, sufficient for 50 gel lanes. Successively larger concatemers of lambda DNA (cI857 ind 1 Sam7) are embedded in 1% LMP agarose. Size range: 48.5–1,018 kb.
\nMidRange PFG Marker consists of concatemers of λ DNA isolated from the bacteriophage λ (cI857 ind1 Sam7) mixed with XhoI digested λ DNA embedded in 1% LMP agarose and supplied in a GelSyringe dispenser. XhoI produces fragments of 15.0 and 33.5 kb. These fragments\nanneal to and form concatemers with intact λ DNA. Size\nrange: 15–291 kb.\n
\nThe Lambda DNA-Mono Cut Mix is best separated by\npulsed field gel electrophoresis, but can be alternatively\nused with standard electrophoresis systems. It is supplied\nin a liquid format. Size range: 1.5–48.5 kb.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"N0341","ProductName":"Lambda PFG Ladder"},{"SortOrder":2,"Sku":"N0342","ProductName":""},{"SortOrder":3,"Sku":"N3019","ProductName":"λ DNA-Mono Cut Mix"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":4,"Category":"Conventional DNA Markers","ProductFamily":"1","ProductFamilyDescription":"NEB offers a wide range of double-stranded DNA molecular weight markers for conventional agarose gel electrophoresis. These standards have a size range of approximately 10–23,000 base pairs.
\nThe typical pattern generated by each of the conventional markers is shown below. The number of fragments generated by each marker, as well as the specific fragment sizes for each of the conventional markers can be found online.
\nUsage Recommendation: Dilution of these markers\nis recommended in TE or other buffer of minimal ionic\nstrength. DNA may denature if diluted in dH2O.\n
\nThe cohesive ends of fragments 1 and 4 of the Lambda\nDNA-HindIII and Lambda DNA-BstEII Digests can be\nseparated by heating to 60°C for 3 minutes.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"N3012","ProductName":"Lambda DNA HindIII Digest"},{"SortOrder":2,"Sku":"N3014","ProductName":"λ DNA-BstEII Digest"},{"SortOrder":3,"Sku":"N3026","ProductName":"ΦX174 DNA-HaeIII Digest"},{"SortOrder":4,"Sku":"N3031","ProductName":"pBR322 DNA-BstNI Digest"},{"SortOrder":5,"Sku":"N3032","ProductName":"pBR322 DNA-MspI Digest"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""}],"Products":{"Products":[{"SortOrder":1,"Sku":"N0472","ProductName":"Supercoiled DNA Ladder"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":3,"Category":"RNA Markers & Ladders","ProductFamily":"1","ProductFamilyDescription":"NEB offers several RNA Markers and Ladders with a size range from 17 to 9,000 bases. The Low Range ssRNA Ladder and the ssRNA Ladder are suitable for use as RNA size standards on denaturing or native gels. Both are supplied with RNA Loading Dye (2X) (NEB #B0363) and feature a higher intensity fragment to serve as a reference band. The microRNA Marker, provided in a ready-to-load denaturing solution, is ideally used as a size marker on denaturing polyacrylamide gels or northern blots and is best visualized stained with SYBR®-Gold. It is supplied with a 3´-biotinylated 21-mer oligonucleotide probe that can be labeled with g32-P-ATP and T4 PNK (NEB #M0201). The dsRNA Ladder is suitable for use as a size standard in dsRNA and RNAi analysis on both polyacrylamide and agarose gels.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"N0363","ProductName":"dsRNA Ladder"},{"SortOrder":2,"Sku":"N2102","ProductName":"microRNA Marker"},{"SortOrder":3,"Sku":"N0362","ProductName":"ssRNA Ladder"},{"SortOrder":4,"Sku":"N0364","ProductName":"Low Range ssRNA Ladder"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":4,"Category":"Protein Standards","ProductFamily":"1","ProductFamilyDescription":"NEB offers a selection of highly pure protein standards available as unstained, blue prestained or color prestained (containing two colored reference bands for easy identification). Sizes range from 10 to 250 kDa which is ideal for calculating molecular weight determination for a wide range of expressed proteins. NEB protein standards provide uniform band intensities, convenient band spacing and easy-to-identify reference bands.
\nRecommended Load Volume: 3 µl
\nNote: For calculating molecular weight determinations, use NEB's Unstained Protein Standard, Broad Range.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"P7717","ProductName":"Unstained Protein Standard, Broad Range (10-200 kDa)"},{"SortOrder":2,"Sku":"P7719","ProductName":"Color Prestained Protein Standard, Broad Range (10-250 kDa)"},{"SortOrder":3,"Sku":"P7718","ProductName":"Blue Prestained Protein Standard, Broad Range (11–250 kDa)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""}],"Products":{"Products":[{"SortOrder":1,"Sku":"B7024","ProductName":"DNA Gel Loading Dye"},{"SortOrder":2,"Sku":"B7025","ProductName":"Gel Loading Dye, Purple (6X), no SDS"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":7,"Category":"Genome Editing","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"Featured NEB Products Supporting CRISPR Workflows","ProductFamily":"1","ProductFamilyDescription":"New England Biolabs provides reagents to support a broad variety of CRISPR/Cas genome editing approaches. From introduction of Cas and single guide RNA (sgRNA) on plasmids, to direct introduction of Cas ribonucleoprotein (RNP) and detection of edits using next generation sequencing or enzymatic mutation detection, NEB provides reagents that simplify and shorten genome editing workflows.","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"M0668","ProductName":"EnGen® Seq1 Cas9"},{"SortOrder":2,"Sku":"M0669","ProductName":"EnGen® SpRY Cas9"},{"SortOrder":3,"Sku":"m0667","ProductName":"EnGen® Spy Cas9 HF1"},{"SortOrder":4,"Sku":"M0646","ProductName":"EnGen® Spy Cas9 NLS"},{"SortOrder":5,"Sku":"E3321","ProductName":"EnGen® Mutation Detection Kit"},{"SortOrder":6,"Sku":"E3322","ProductName":"EnGen® sgRNA Synthesis Kit, S. pyogenes"},{"SortOrder":7,"Sku":"M0650","ProductName":"EnGen® Spy Cas9 Nickase"},{"SortOrder":8,"Sku":"M0652","ProductName":"EnGen® Spy dCas9 SNAP tag"},{"SortOrder":9,"Sku":"M0653","ProductName":"EnGen® Lba Cas12a (Cpf1)"},{"SortOrder":10,"Sku":"M0654","ProductName":"EnGen® Sau Cas9"},{"SortOrder":11,"Sku":"M0386","ProductName":"Cas9 Nuclease, S. pyogenes"},{"SortOrder":12,"Sku":"T2040","ProductName":"Monarch® Spin RNA Cleanup Kit (50 µg)"},{"SortOrder":13,"Sku":"E0554","ProductName":"Q5® Site-Directed Mutagenesis Kit"},{"SortOrder":14,"Sku":"E0552","ProductName":"Q5® Site-Directed Mutagenesis Kit (Without Competent Cells)"},{"SortOrder":15,"Sku":"E2621","ProductName":"NEBuilder® HiFi DNA Assembly Master Mix"},{"SortOrder":16,"Sku":"E5520","ProductName":"NEBuilder® HiFi DNA Assembly Cloning Kit"},{"SortOrder":17,"Sku":"E2080","ProductName":"HiScribe® T7 mRNA Kit with CleanCap® Reagent AG ..."},{"SortOrder":18,"Sku":"E2060","ProductName":"HiScribe® T7 ARCA mRNA Kit (with tailing)"},{"SortOrder":19,"Sku":"E2065","ProductName":"HiScribe® T7 ARCA mRNA Kit"},{"SortOrder":20,"Sku":"E2040","ProductName":"HiScribe® T7 High Yield RNA Synthesis Kit"},{"SortOrder":21,"Sku":"E2050","ProductName":"HiScribe® T7 Quick High Yield RNA Synthesis Kit"},{"SortOrder":22,"Sku":"M0302","ProductName":"T7 Endonuclease I"},{"SortOrder":23,"Sku":"M0689","ProductName":"Authenticase®"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"1","DisplayAsTableColumns":[{"FieldName":"FullProductName","ColumnDisplayName":"Product"},{"FieldName":"CatalogNumber","ColumnDisplayName":"NEB #"},{"FieldName":"Size","ColumnDisplayName":"Size"},{"FieldName":"CatalogApplications","ColumnDisplayName":"Applications"}],"GroupFieldName":""},{"SortOrder":2,"Category":"Programmable Nucleases","ProductFamily":"1","ProductFamilyDescription":"The highest efficiency strategy for genome editing with CRISPR/Cas nucleases is direct introduction of Cas/guide RNA complexes. This method further simplifies CRISPR/Cas workflows and has been reported to increase on-target editing activity and reduce off-target events. NEB provides purified Cas9 nucleases from S. pyogenes, S. aureus, and S. equinus, and Cas12a (Cpf1) nuclease from Lachnospiraceae bacterium ND2006. In addition, NEB provides variants of Cas9 from S. pyogenes, including nicking endonuclease and endonuclease deficient versions. NEB also provides Thermus thermophilus argonaute (TtAgo), a programmable DNA endonuclease which requires a short 5´-phosphorylated single-stranded DNA guide to target its activity to a specific corresponding sequence on a substrate.","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"M0668","ProductName":"EnGen® Seq1 Cas9"},{"SortOrder":2,"Sku":"M0669","ProductName":"EnGen® SpRY Cas9"},{"SortOrder":3,"Sku":"m0667","ProductName":"EnGen® Spy Cas9 HF1"},{"SortOrder":4,"Sku":"M0646","ProductName":"EnGen® Spy Cas9 NLS"},{"SortOrder":5,"Sku":"M0650","ProductName":"EnGen® Spy Cas9 Nickase"},{"SortOrder":6,"Sku":"M0386","ProductName":"Cas9 Nuclease, S. pyogenes"},{"SortOrder":7,"Sku":"M0652","ProductName":"EnGen® Spy dCas9 SNAP tag"},{"SortOrder":8,"Sku":"M0653","ProductName":"EnGen® Lba Cas12a (Cpf1)"},{"SortOrder":9,"Sku":"M0654","ProductName":"EnGen® Sau Cas9"},{"SortOrder":10,"Sku":"M0665","ProductName":"Tth Argonaute (TtAgo)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"1","DisplayAsTableColumns":[{"FieldName":"FullProductName","ColumnDisplayName":"Product"},{"FieldName":"CatalogNumber","ColumnDisplayName":"NEB #"},{"FieldName":"Size","ColumnDisplayName":"Size"},{"FieldName":"CatalogFeatures","ColumnDisplayName":"Features"}],"GroupFieldName":""}],"Products":{"Products":[{"SortOrder":1,"Sku":"E3321","ProductName":"EnGen® Mutation Detection Kit"},{"SortOrder":2,"Sku":"E3322","ProductName":"EnGen® sgRNA Synthesis Kit, S. pyogenes"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":8,"Category":"RNA Reagents","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"RNA Synthesis","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"HiScribe® T7 High Yield RNA Synthesis Kits","ProductFamily":"1","ProductFamilyDescription":"Description: NEB's HiScribe T7 High Yield RNA Synthesis Kits offer robust in vitro RNA transcription of many kinds of RNA, including internally labeled and co-transcriptionally capped RNA. Utilizing T7 RNA Polymerase, these kits achieve efficient transcription with small amounts of template, and can generate up to 180 µg per reaction, or up to 30–40 µg of capped RNA using a cap analog. RNA generated can be used in a variety of applications, including RNA structure/function studies, ribozyme biochemistry, probes for RNase protection assays and hybridization-based blots, anti-sense RNA and RNAi experiments, microarray analysis, microinjection, and in vitro translation and RNA vaccines.
\nThe HiScribe T7 High Yield RNA Synthesis Kit is an extremely flexible system, with separate NTPs included for flexible reaction setup. The HiScribe T7 Quick High Yield RNA Synthesis Kit is provided in master mix format for fast reaction setup. Each kit can yield up to 9 mg of RNA.
\nThe HiScribe T7 High Yield RNA\nSynthesis Kit Includes:
\nThe HiScribe T7 Quick High Yield RNA\nSynthesis Kit Includes:
\nDescription: Most eukaryotic mRNAs require a N7-methyl guanosine (m7G) cap structure at the 5´ end and a poly(A) tail at the 3´ end to be efficiently translated. By using a DNA template encoding a poly(A) tail, the HiScribe T7 ARCA mRNA Kit can be used to synthesize capped and tailed mRNAs. The cap structure is added to the mRNA by co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA, NEB #S1411) using T7 RNA Polymerase. The transcription reaction can be set up easily by combining the ARCA/NTP Mix, T7 RNA Polymerase Mix and a suitable DNA template. The kit also allows for partial incorporation of 5mCTP, Pseudo-UTP, N1-Methyl-Pseudo-UTP, 5-Methoxy-UTP and other modified nucleotides into mRNA. mRNAs synthesized with the kit can be used for cell transfection, microinjection, in vitro translation and RNA vaccines. Poly(A) tail is incorporated during the transcription reaction. The kit also includes DNase I and LiCl for DNA template removal and quick mRNA purification.
\nThe HiScribe T7 ARCA mRNA Kit (with tailing) is designed for quick production of ARCA capped and poly(A) tailed mRNA in vitro from templates without encoded poly(A) tails.
\nThe HiScribe T7 ARCA mRNA Kit Includes:
\nThe HiScribe T7 ARCA mRNA Kit\n(with tailing) Includes:
\nAdvantages:
\nDescription: Initiation of transcription with T3, T7 and SP6 RNA Polymerases is highly specific for the T3, T7 and SP6 phage promoters, respectively. Cloning vectors have been developed which direct transcription from the T3, T7 or SP6 promoter through polylinker cloning sites. These vectors allow in vitro synthesis of defined RNA transcripts from a cloned DNA sequence. T7 RNA Polymerase (High Concentration) is offered at a 20-fold higher concentration than our standard T7 RNA Polymerase and is ideal for experienced users interested in building and optimizing their own in vitro transcription reactions.
\nHi-T7 RNA Polymerase is an engineered, thermoactive T7 RNA Polymerase. Hi-T7 uses T7 RNA Polymerase promoters. It can increase capping efficiency and\neliminate dsRNA by-product formation during synthesis.\nHi-T7 RNA Polymerase (High Concentration) is offered at\na 20-fold higher concentration than our standard Hi-T7\nRNA Polymerase and is ideal for experienced users\ninterested in building and optimizing their own in vitro\ntranscription reactions.\n
\nReaction Conditions: 1X RNAPol Reaction Buffer.\nSupplement with 0.5 mM each ATP, UTP, GTP, CTP\n(not included) and DNA template containing the appropriate promoter. Incubate at 37°C (T3, T7 and SP6)\nor 50°C (Hi-T7). Protocols involving high concentration\nT7 and Hi-T7 RNA Polymerases are to be designed and\noptimized by the user.\n
\nUnit Definition: One unit is defined as the amount of\nenzyme required to incorporate 1 nmol ATP into an acidinsoluble material in 1 hour at 37°C or 50°C for Hi-T7.\nUnit assay conditions can be found at www.neb.com.\n
\nConcentration: T3 RNA Polymerase: 50,000 units/ml.\nT7 RNA Polymerase: 50,000 units/ml. T7 RNA Polymerase\n(High Concentration): 1,000,000 units/ml. SP6 RNA Polymerase: 20,000 units/ml. Hi-T7 RNA Polymerase: 50,000\nunits/ml. Hi-T7 RNA Polymerase (High Concentration):\n1,000,000 units/ml.
","ProductFamilyIconCharacters":["r"],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"M0378","ProductName":"T3 RNA Polymerase"},{"SortOrder":2,"Sku":"M0251","ProductName":"T7 RNA Polymerase"},{"SortOrder":3,"Sku":"M0460","ProductName":"T7 RNA Polymerase (High Concentration)"},{"SortOrder":4,"Sku":"M0207","ProductName":"SP6 RNA Polymerase"},{"SortOrder":5,"Sku":"M0658","ProductName":"Hi-T7® RNA Polymerase"},{"SortOrder":6,"Sku":"M0470","ProductName":"Hi-T7® RNA Polymerase (High Concentration)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":4,"Category":"E. coli RNA Polymerase, Core Enzyme & Holoenzyme","ProductFamily":"1","ProductFamilyDescription":"Description: E. coli RNA Polymerase Core Enzyme\nconsists of 5 subunits designated α, α, β´, β, and ω.\nThe enzyme is free of sigma factor and does not initiate\nspecific transcription from bacterial and phage DNA promoters. The enzyme retains the ability to transcribe RNA\nfrom nonspecific initiation sequences. Addition of sigma\nfactors will allow the enzyme to initiate RNA synthesis\nfrom specific bacterial and phage promoters. The core\nenzyme has a molecular weight of ~ 400 kDa.\n
\nE. coli RNA Polymerase Holoenzyme is the core enzyme\nsaturated with sigma factor 70. The Holoenzyme initiates\nRNA synthesis from sigma 70 specific bacterial and\nphage promoters.\n
\nReaction Conditions: 1X E. coli RNA Polymerase\nReaction Buffer, 0.5 mM of each rNTP and DNA template.\nIncubate at 37°C.\n
\nUnit Definition: One unit is the amount of enzyme\nrequired to incorporate 1 nmol of NTP into RNA in\n10 minutes at 37°C. Unit assay conditions can be found\nat www.neb.com.\n
\nConcentration: 1,000 units/ml
","ProductFamilyIconCharacters":["w"],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"M0550","ProductName":"E. coli RNA Polymerase, Core Enzyme"},{"SortOrder":2,"Sku":"M0551","ProductName":"E. coli RNA Polymerase, Holoenzyme"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":5,"Category":"Pyrophosphatases","ProductFamily":"1","ProductFamilyDescription":"Description: Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate.
\nP207–4 + H20 → 2HP04–2
\nSource: Pyrophosphatase, Inorganic (E. coli) is prepared from a clone of the E. coli inorganic pyrophosphatase gene.
\nPyrophosphatase, Inorganic (yeast) is from an E. coli strain containing a genetic fusion of the Saccharomyces cerevisiae ppa gene and the gene coding for Mycobacterium xenopi GyrA intein. Developed by BioHelix Corporation, now a wholly owned subsidiary of Quidel Corporation, and produced at New England Biolabs.
\nThermostable Inorganic Pyrophosphatase is from an E. coli strain carrying a plasmid encoding a pyrophosphatase from the extreme thermophile Thermococcus litoralis.
\nUnit Definition: One unit is defined as the amount\nof enzyme that will generate 1 µmol of phosphate per\nminute from inorganic pyrophosphate under standard\nreaction conditions.
\nConcentration: Pyrophosphatase, Inorganic\n(E. coli) and Pyrophosphatase, Inorganic (yeast): 100 units/ml. Thermostable Inorganic Pyrophosphatase: 2,000 units/ml. NudC Pyrophosphatase: 10 µM
","ProductFamilyIconCharacters":["r","9"],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"M0361","ProductName":"Pyrophosphatase, Inorganic (E. coli)"},{"SortOrder":2,"Sku":"M2403","ProductName":"Pyrophosphatase, Inorganic (yeast)"},{"SortOrder":3,"Sku":"M0296","ProductName":"Thermostable Inorganic Pyrophosphatase"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":6,"Category":"Ribonucleotides","ProductFamily":"1","ProductFamilyDescription":"Description: Ribonucleotide Solution Set consists of four separate solutions of ATP, GTP,\nCTP and UTP, pH 7.5, as sodium salts. Each nucleotide\nis supplied at 100 mM.
\nRibonucleotide Solution Mix consists of a buffered equimolar solution of ribonucleotide triphosphates (ATP, CTP, GTP and UTP), pH 7.5, as sodium\nsalts. Each nucleotide is supplied at a concentration of\n25 mM (total NTP concentration equals 100 mM).
\nModified NTPs are commonly used for reduction of immunogenicity for in vitro transcription RNA. 5-Methyl-Cytidine-5'-Triphosphoate (5-Methyl-CTP) is supplied as sodium salt, pH 7.3. Pseudouridine-5’-Triphosphate (Pseudo-UTP) is supplied as sodium salt, pH 7.0. N1-Methyl-Pseudouridine-5’-Triphosphate (N1-Methyl-Pseudo-UTP) is supplied as sodium salt, pH 7.5. 5-Methoxy-Uridine-5’-Triphosphate (5-Methoxy-UTP) is supplied as sodium salt, pH 7.1. All modified NTPs are supplied at a concentration of 100 mM.
\nNote: To ensure maximum activity upon long-term\nstorage, aliquot and store at –80°C
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"N0450","ProductName":"Ribonucleotide Solution Set"},{"SortOrder":2,"Sku":"N0466","ProductName":"Ribonucleotide Solution Mix"},{"SortOrder":3,"Sku":"N0431","ProductName":"N1-Methyl-Pseudouridine-5´-Triphosphate (N1-Methyl-Pseudo-UTP)"},{"SortOrder":4,"Sku":"N0432","ProductName":"5-Methyl-Cytidine-5´-Triphosphate (5-Methyl-CTP)"},{"SortOrder":5,"Sku":"N0433","ProductName":"Pseudouridine-5´-Triphosphate (Pseudo-UTP)"},{"SortOrder":6,"Sku":"N0434","ProductName":"5-Methoxy-Uridine-5´-Triphosphate (5-Methoxy-UTP)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":7,"Category":"RNA Cap Analog Selection Chart","ProductFamily":"1","ProductFamilyDescription":"The 5´ terminal m7G cap present on most eukaryotic mRNAs is required for translation, in vitro, at the initiation level. For most RNAs, the cap structure increases stability, decreases susceptibility to exonuclease degradation, and promotes the formation of mRNA initiation complexes. Certain prokaryotic mRNAs with 5´ terminal cap structures are translated as efficiently as eukaryotic mRNA in a eukaryotic cell-free protein synthesizing system. Splicing of certain eukaryotic substrate RNAs has also been observed to require a cap structure.","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"S1411","ProductName":"3´-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog"},{"SortOrder":2,"Sku":"S1404","ProductName":"m7G(5')ppp(5')G RNA Cap Structure Analog"},{"SortOrder":3,"Sku":"S1407","ProductName":"G(5')ppp(5')G RNA Cap Structure Analog"},{"SortOrder":4,"Sku":"S1405","ProductName":"m7G(5')ppp(5')A RNA Cap Structure Analog"},{"SortOrder":5,"Sku":"S1406","ProductName":"G(5')ppp(5')A RNA Cap Structure Analog"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"1","DisplayAsTableColumns":[{"FieldName":"FullProductName","ColumnDisplayName":"Product"},{"FieldName":"CatalogNumber","ColumnDisplayName":"NEB #"},{"FieldName":"Size","ColumnDisplayName":"Size"},{"FieldName":"CatalogApplications","ColumnDisplayName":"Applications"}],"GroupFieldName":""},{"SortOrder":8,"Category":"3´-Desthiobiotin-GTP & 3´-Biotin-GTP","ProductFamily":"1","ProductFamilyDescription":"Description: 3´-Desthiobiotin-GTP or 3´-Biotin GTP are guanosine triphosphate (GTP) analogs which are modified at their 3´ position with desthiobiotin or biotin, respectively. When used with the Vaccinia Capping System, (NEB #M2080) these reagents enable affinity tagging of RNA triphosphate ends. Tagged RNAs are enriched by binding to Hydrophilic Streptavidin Magnetic Beads (NEB #S1421). Desthiobiotin-tagged RNAs can be eluted with free biotin. This approach is used in Cappable-seq, a method developed at NEB for directly enriching the 5´ ends of primary transcripts (1).
\nReference:
\n(1) Ettwiller, L. et al. (2016) BMC Genomics, 17,199.
LunaScript RT SuperMix Kit is an optimized master mix containing all the necessary components for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow. It features the thermostable Luna Reverse Transcriptase, which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is also included to protect template RNA from degradation. The LunaScript RT SuperMix Kit contains random hexamer and poly-dT primers, allowing for even coverage across the length of the RNA targets.
\nLunaScript RT Master Mix Kit (Primer-free) is the same formulation as the LunaScript RT SuperMix Kit but does not contain primers. It includes all components needed for carrying out first strand cDNA synthesis except for RNA template and user-supplied primers. It provides flexible options for using different primers (dT primers, random primers, gene-specific primers, modified primers, etc.) for first strand cDNA synthesis.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"M3010","ProductName":"LunaScript® RT SuperMix"},{"SortOrder":2,"Sku":"E3010","ProductName":"LunaScript® RT SuperMix Kit"},{"SortOrder":3,"Sku":"E3025","ProductName":"LunaScript® RT Master Mix Kit (Primer-free)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":3,"Category":"WarmStart® RTx Reverse Transcriptase","ProductFamily":"1","ProductFamilyDescription":"Description: WarmStart RTx Reverse Transcriptase is a unique in silico-designed, RNA-directed DNA polymerase coupled with a reversibly-bound aptamer that inhibits RTx activity below 40°C. This enzyme can synthesize a complementary DNA strand initiating prom a primer using RNA (cDNA synthesis) or single-stranded DNA as a template. RTx is a robust enzyme for RNA detection in amplification reactions and is particularly well suited for use in Loop-mediated Isothermal Amplification (LAMP). The WarmStart property enables high-throughput applications, room-temperature setup, and increases consistency and specificity of amplification reactions. RTx contains intact RNase H activity. The glycerol-free version of WarmStart RTx Reverse Transcriptase supports lyophilization and automated workflows.
\nReaction Conditions: 1X Isothermal Amplification Buffer (or 1X Isothermal Amplification Buffer (Lyo-compatible)), template, primer, dNTPs and 0.25-0.5 μl of WarmStart RTx Reverse Transcriptase in a reaction volume of 25 μl. Incubate at 50-55°C for cDNA synthesis or directly at 65°C for RT-LAMP. Heat inactivation: 80°C for 10 minutes.
\nUnit Definition: One unit is defined as the amount of enzyme that will incorporate 1nmol of dTTP into acid-insoluble material in a total reaction volume of 50 μl in 20 minutes at 50°C using poly(rA) - oligo(dT)18 as a template.
\nConcentration: WarmStart RTx Reverse Transcriptase: 15,000 units/ml. WarmStart RTx Reverse Transcriptase (Glycerol-free): 75,000 units/ml.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"M0380","ProductName":"WarmStart® RTx Reverse Transcriptase"},{"SortOrder":2,"Sku":"M0439","ProductName":"WarmStart® RTx Reverse Transcriptase (Glycerol-free)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":4,"Category":"Primers for cDNA Synthesis","ProductFamily":"1","ProductFamilyDescription":"Oligo d(N)n primers are used for the priming and sequencing of mRNA adjacent to the 3´-poly A tail or tailed cDNA. Note: #S1316 does not contain a 5´-phosphate.","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"S1230","ProductName":"Random Primer 6"},{"SortOrder":2,"Sku":"S1254","ProductName":"Random Primer 9"},{"SortOrder":3,"Sku":"S1327","ProductName":"Oligo d(T)23 VN"},{"SortOrder":4,"Sku":"S1330","ProductName":"Random Primer Mix"},{"SortOrder":5,"Sku":"S1316","ProductName":"Oligo d(T)18 mRNA Primer"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"1","DisplayAsTableColumns":[{"FieldName":"FullProductName","ColumnDisplayName":"Product"},{"FieldName":"CatalogNumber","ColumnDisplayName":"NEB #"},{"FieldName":"Size","ColumnDisplayName":"Size"}],"GroupFieldName":""},{"SortOrder":5,"Category":"RT-PCR & RT-qPCR Kits","ProductFamily":"1","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E3005","ProductName":"Luna® Universal One-Step RT-qPCR Kit"},{"SortOrder":2,"Sku":"E3006","ProductName":"Luna® Universal Probe One-Step RT-qPCR Kit"},{"SortOrder":3,"Sku":"E3007","ProductName":"Luna® Probe One-Step RT-qPCR Kit (No ROX)"},{"SortOrder":4,"Sku":"E3030","ProductName":"Luna® Cell Ready One-Step RT-qPCR Kit"},{"SortOrder":5,"Sku":"E3031","ProductName":"Luna® Cell Ready Probe One-Step RT-qPCR Kit"},{"SortOrder":6,"Sku":"M3019","ProductName":"Luna® Probe One-Step RT-qPCR 4X Mix with UDG"},{"SortOrder":7,"Sku":"M3029","ProductName":"Luna® Probe One-Step RT-qPCR 4X Mix with UDG (No ROX)"},{"SortOrder":8,"Sku":"E3019","ProductName":"Luna® SARS-CoV-2 RT-qPCR Multiplex Assay Kit"},{"SortOrder":9,"Sku":"E1555","ProductName":"LunaScript® Multiplex One-Step RT-PCR Kit"},{"SortOrder":10,"Sku":"E5315","ProductName":"OneTaq® One-Step RT-PCR Kit"},{"SortOrder":11,"Sku":"E5310","ProductName":"OneTaq® RT-PCR Kit"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""}],"Products":{"Products":[{"SortOrder":1,"Sku":"M0368","ProductName":"ProtoScript® II Reverse Transcriptase"},{"SortOrder":2,"Sku":"M0253","ProductName":"M-MuLV Reverse Transcriptase"},{"SortOrder":3,"Sku":"M0277","ProductName":"AMV Reverse Transcriptase"},{"SortOrder":4,"Sku":"M0681","ProductName":"Induro® Reverse Transcriptase"},{"SortOrder":5,"Sku":"M0466","ProductName":"Template Switching RT Enzyme Mix"},{"SortOrder":6,"Sku":"S1230","ProductName":"Random Primer 6"},{"SortOrder":7,"Sku":"S1254","ProductName":"Random Primer 9"},{"SortOrder":8,"Sku":"S1327","ProductName":"Oligo d(T)23 VN"},{"SortOrder":9,"Sku":"S1330","ProductName":"Random Primer Mix"},{"SortOrder":10,"Sku":"S1316","ProductName":"Oligo d(T)18 mRNA Primer"},{"SortOrder":11,"Sku":"E6560","ProductName":"ProtoScript® II First Strand cDNA Synthesis Kit"},{"SortOrder":12,"Sku":"E6300","ProductName":"ProtoScript® First Strand cDNA Synthesis Kit"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":3,"Category":"RNA Ligases & Modifying Enzymes","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"T4 RNA Ligase 1 (ssRNA Ligase)","ProductFamily":"1","ProductFamilyDescription":"Description: Catalyzes ligation of a 5´ phosphorylterminated nucleic acid donor to a 3´ hydroxyl-terminated nucleic acid acceptor through the formation of a 3´→ 5´ phosphodiester bond with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates.
\nSource: An E. coli strain that carries the T4 RNA Ligase 1 gene
\nReaction Conditions: 1X T4 RNA Ligase Reaction Buffer, 25°C. Supplement with 1 mM ATP (included). Heat Inactivation: 65°C for 15 minutes.
\nNotes on Use: Addition of DMSO to 10% (v/v) is\nrequired for pCp ligation.\n
\nReagents Supplied:
\n10X T4 RNA Ligase Reaction Buffer
\n10 mM ATP (with NEB #M0204) or 100 mM ATP (with\nNEB #M0437)
\n50% PEG 8000
\nUnit Definition: One unit is defined as the amount of\nenzyme required to convert 1 nmol of 5´-[32P] rA16 into\na phosphate resistant form in 30 minutes at 37°C.
\nConcentration: 10,000 or 30,000 units/ml
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\nMutation of K227 in T4 RNA Ligase 2 reduces enzyme lysyl adenylation. K227Q reduces the formation of undesired ligation products (concatemers and circles) by T4 Rnl2tr. It does so by reducing the trace activity of T4 Rnl2tr in transfer of adenylyl groups from linkers to the 5´-phosphates of input RNAs. T4 Rnl2tr KQ is a double-point mutant of T4 RNA Ligase 2, truncated (NEB #M0242). Mutation of R55K in T4 Rnl2tr K227Q increases the ligation activity of the enzyme to levels similar to T4 Rnl2tr.
\nThe exclusion of ATP, use of pre-adenylated linkers, and the reduced enzyme lysyl adenylation activity provide the lowest possible background in ligation reactions.These enzymes have been used for optimized linker ligation for high-throughput sequencing library construction of small RNAs.
\nSource: Expressed as an MBP fusion from a plasmid in\nE. coli which encodes the first 249 amino acids of the full\nlength T4 RNA Ligase 2. T4 Rnl2tr K227Q has a lysine\nto glutamine mutation at position 227. T4 Rnl2tr KQ has\nan arginine to lysine and lysine to glutamine mutation at\npositions 55 and 227, respectively.\n
\nReaction Conditions: 1X T4 RNA Ligase Reaction\nBuffer, 25°C. Heat inactivation: 65°C for\n20 minutes.\n
\nReagents Supplied:
\nUnit Definition: 200 units is defined as the amount of\nenzyme required to give 80% ligation of a 31-mer RNA\nto the pre-adenylated end of a 17-mer DNA [Universal\nmiRNA Cloning Linker (NEB #S1315)] in a total reaction\nvolume of 20 µl in 1 hour at 25°C.
\nConcentration: 200,000 units/ml
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\nDNase I-XT is a salt tolerant enzyme that exhibits optimal activity between 50-100 mM salt and retains 65% and ~40% activity in 200 and 300 mM salt, respectively. Increased salt tolerance makes it ideal for DNA template removal from an in vitro transcription (IVT) reaction.
\nBoth enzymes are RNase-free, allowing for complete removal of DNA from RNA preps, while maintaining RNA integrity.
\nReaction Conditions: 1X DNase I Reaction Buffer, 37°C. DNase I can be heat inactivated at 75°C for 10 minutes, while DNase I-XT cannot.
\nReagents Supplied:
\nUnit Definition: DNase I – One unit is defined as the amount of enzyme which will completely degrade 1 µg of pBR322 DNA in a total reaction volume of 50 µl in 10 minutes at 37°C. DNase I-XT – One unit is defined as the amount of enzyme required to release 210 pmol of FAM from a 35-mer FAM-BHQ1 labeled hairpin oligonucleotide in 1 minute at 30°C in a 50 µl reaction volume.
\nConcentration: 2,000 units/ml
\nNotes: DNase I-XT is supplied with an optimized reaction buffer. Use with supplied buffer, and not DNase I Reaction Buffer. Likewise, due to the sub-optimal salt concentration, do not use DNase I-XT Buffer with DNase I. When using DNase I, EDTA should be added to a final concentration of 5 mM to protect RNA from being degraded during enzyme inactivation.
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\nDescription: The Monarch Spin RNA Isolation Kit (Mini) is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from various biological samples, including cultured cells, mammalian tissues, microbes, plants, insects and blood. With just this single kit, users can purify up to 100 µg of high-quality total RNA from multiple sample types, from standard as well as tough-to-lyse samples, with included enzymes, reagents, and specialized gDNA removal columns. The kit uniquely enables binding capacities like RNA purification miniprep kits, combined with the low elution volumes of micro kits. Cleanup of enzymatic reactions and purification of RNA from TRIzol® -extracted samples is also possible using this kit. Purified RNA has metrics with A260/280 and A260/230 ratios typically > 2.0, high RNA integrity scores, and minimal residual gDNA. This kit captures RNA ranging in size from full-length rRNAs down to intact miRNAs. Purified RNA is suitable for downstream applications such as RT-qPCR, cDNA synthesis, RNA-seq, and RNA hybridization-based technologies.
\nThe Monarch Spin RNA Cleanup Kits provide a fast and simple silica spin column-based solution for RNA cleanup and concentration after any enzymatic reaction (including in vitro transcription, DNase I treatment, capping and labeling) and after other purification methods such as phenol/chloroform extraction. These kits can also be used to extract total RNA from cells, saliva and buccal/ nasopharyngeal swabs. The Monarch Spin RNA Cleanup Kits are available in 3 different binding capacities (10 μg, 50 μg and 500 μg). Each kit contains unique columns, all designed to prevent buffer retention and ensure no carryover of contaminants, enabling low-volume elution\nof highly-pure RNA. Following the standard protocol,\nRNA ≥ 25 nt is purified with this kit; however, a modified\nprotocol is available to enable the binding of RNA as\nsmall as 15 nt (including miRNAs).
\nThe Monarch Spin RNA Isolation Kit (Mini) Includes:
\nThe Monarch Spin RNA Cleanup Kits Include:
\nNEB offers several RNA Markers and Ladders with a size range from 17 to 9,000 bases. The ssRNA ladders are suitable for use as RNA size standards on denaturing or native gels. Both are supplied with 2X Loading Buffer and feature a higher intensity fragment to serve as a reference band. The microRNA Marker, supplied in a readyto-load denaturing solution, is ideally used as a size marker on polyacrylamide gels or Northern blots and is best visualized stained with ssRNA fluorescent dyes. It is supplied with a 3´-biotinylated 21-mer oligonucleotide\nprobe that can also be labeled with [φ-32P] ATP and T4\nPNK (NEB #M0201). The dsRNA Ladder is suitable\nfor use as a size standard in dsRNA analysis on both\npolyacrylamide and agarose gels.\n
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"N0363","ProductName":"dsRNA Ladder"},{"SortOrder":2,"Sku":"N2102","ProductName":"microRNA Marker"},{"SortOrder":3,"Sku":"N0362","ProductName":"ssRNA Ladder"},{"SortOrder":4,"Sku":"N0364","ProductName":"Low Range ssRNA Ladder"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""}],"Products":{"Products":[{"SortOrder":1,"Sku":"B0363","ProductName":"RNA Loading Dye, (2X)"},{"SortOrder":2,"Sku":"S1315","ProductName":"Universal miRNA Cloning Linker"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":8,"Category":"Other","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"B0363","ProductName":"RNA Loading Dye, (2X)"},{"SortOrder":2,"Sku":"S1315","ProductName":"Universal miRNA Cloning Linker"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""}],"Products":{"Products":[]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":9,"Category":"Protein Expression & Purification","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"Cell-Free Expression","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"PURExpress® In Vitro Protein Synthesis Kits","ProductFamily":"1","ProductFamilyDescription":"Description: A rapid method for gene expression analysis, PURExpress is a novel cell-free transcription/ translation system reconstituted from the purified components necessary for E. coli translation. The minimized nuclease activity and protease-free nature of the PURExpress platform preserves the integrity of DNA and RNA templates/complexes and results in proteins that are free of modification and degradation. Transcription and translation are carried out in a one-step reaction, and require the mixing of only two tubes. With results available in a few hours, PURExpress saves valuable laboratory time and is ideal for high-throughput technologies.
\nAdvantages:
\nPURExpress Disulfide Bond Enhancer: This proprietary blend of proteins and buffer components is designed to correctly fold target proteins with multiple disulfide bonds produced in PURExpress reactions or NEBExpress E. coli S30 extracts. Added at the beginning of a reaction, the components assist with the oxidation of cysteine thiols and correcting mis-oxidized substrates, increasing yield of soluble and functionally active protein.
\nPURExpress ∆ Ribosome Kit: The ribosomes are\nremoved from this kit, allowing users to add their own\nmodified ribosomes for studies on protein translation. Control ribosomes (sufficient for 2 reactions) are\nprovided in a separate tube.\n
\nPURExpress ∆ RF123 Kit: Release factors are involved in termination of protein translation by recognizing\nthe stop codons in an mRNA sequence. In a ribosome\ndisplay experiment using PURExpress, lack of release\nfactors could stabilize the ternary complex of mRNA-ribosome-nascent protein. As a result, the cDNA recovery\ncould be higher. In this kit, the three release factors are\nsupplied separately, allowing users to perform a protein\nsynthesis reaction/ribosome display experiment with/\nwithout release factors of their choice.\n
\nPURExpress ∆ (aa, tRNA) Kit: The tRNA and amino\nacids are supplied separately in this kit, allowing users\nto run a protein synthesis reaction by adding modified\namino acids and tRNA mixtures to the reaction.\n
\nE. coli Ribosome: The 70S E. coli Ribosome consists\nof a small subunit (30S) and a large subunit (50S).\nThis preparation of ribosomes is highly active in NEB’s\nPURExpress Protein Synthesis Kit (NEB #E6800), and\ncan be used in ribosome structure and function studies,\nas a target for drug screening, and as starting material for\nisolation of native ribosomal RNAs (5S, 16S, 23S).
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E6800","ProductName":"PURExpress® In Vitro Protein Synthesis Kit"},{"SortOrder":2,"Sku":"E3313","ProductName":"PURExpress® Δ Ribosome Kit"},{"SortOrder":3,"Sku":"E6840","ProductName":"PURExpress® Δ (aa, tRNA) Kit"},{"SortOrder":4,"Sku":"E6850","ProductName":"PURExpress® Δ RF123 Kit"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""}],"Products":{"Products":[{"SortOrder":1,"Sku":"E5360","ProductName":"NEBExpress® Cell-free E. coli Protein Synthesis System"},{"SortOrder":2,"Sku":"E6820","ProductName":"PURExpress® Disulfide Bond Enhancer"},{"SortOrder":3,"Sku":"P0763","ProductName":"E. coli Ribosome"},{"SortOrder":4,"Sku":"P0774","ProductName":"NEBExpress® GamS Nuclease Inhibitor"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":2,"Category":"E. coli","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E8201","ProductName":"NEBExpress® MBP Fusion and Purification System "},{"SortOrder":2,"Sku":"E8021","ProductName":"Amylose Resin"},{"SortOrder":3,"Sku":"E8032","ProductName":"Anti-MBP Monoclonal Antibody"},{"SortOrder":4,"Sku":"N0378","ProductName":"pMAL-c6T Vector"},{"SortOrder":5,"Sku":"P8112","ProductName":"TEV Protease"},{"SortOrder":6,"Sku":"E6901","ProductName":"IMPACT™ Kit"},{"SortOrder":7,"Sku":"N6707","ProductName":"pTXB1 Vector"},{"SortOrder":8,"Sku":"N6709","ProductName":"pTYB21 Vector"},{"SortOrder":9,"Sku":"S6651","ProductName":"Chitin Resin"},{"SortOrder":10,"Sku":"E8034","ProductName":"Anti-CBD Monoclonal Antibody"},{"SortOrder":11,"Sku":"N6951","ProductName":"pTWIN1 Vector"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":3,"Category":"Yeast","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E1000","ProductName":"K. lactis Protein Expression Kit"},{"SortOrder":2,"Sku":"R0157","ProductName":"SacII"},{"SortOrder":3,"Sku":"B9017","ProductName":"Yeast Carbon Base Medium Powder"},{"SortOrder":4,"Sku":"C1001","ProductName":"K. lactis GG799 Competent Cells"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":4,"Category":"Competent Cells for Protein Expression","ProductFamily":"1","ProductFamilyDescription":"NEB offers a wide selection of competent cell strains designed for the expression of a variety of proteins. Proteins with multiple disulfide bonds are correctly oxidized to significantly higher yields with SHuffle® strains. Tunable T7 expression is achieved with Lemo21(DE3), an ideal strain for difficult targets including membrane proteins. NiCo21(DE3) is designed for expression and purification of His-tagged proteins. NEBExpress and T7 Express are offered with varying levels of control. Only NEB offers exceptional control of T7 expression by the lysY gene, which is ideal for proteins that are difficult to express or toxic to the cell. Each strain is provided with a protocol for optimal expression.","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"C2523","ProductName":"NEBExpress® Competent E. coli (High Efficiency)"},{"SortOrder":2,"Sku":"C3037","ProductName":"NEBExpress® Iq Competent E. coli (High Efficiency)"},{"SortOrder":3,"Sku":"C2566","ProductName":"T7 Express Competent E. coli (High Efficiency)"},{"SortOrder":4,"Sku":"C3010","ProductName":"T7 Express lysY Competent E. coli (High Efficiency)"},{"SortOrder":5,"Sku":"C3013","ProductName":"T7 Express lysY/Iq Competent E. coli (High Efficiency)"},{"SortOrder":6,"Sku":"C3028","ProductName":"SHuffle® Express Competent E. coli"},{"SortOrder":7,"Sku":"C3029","ProductName":"SHuffle® T7 Express Competent E. coli"},{"SortOrder":8,"Sku":"C3030","ProductName":"SHuffle® T7 Express lysY Competent E. coli"},{"SortOrder":9,"Sku":"C3026","ProductName":"SHuffle® T7 Competent E. coli"},{"SortOrder":10,"Sku":"C2530","ProductName":"BL21 Competent E. coli"},{"SortOrder":11,"Sku":"C2527","ProductName":"BL21(DE3) Competent E. coli"},{"SortOrder":12,"Sku":"C2528","ProductName":"Lemo21(DE3) Competent E. coli"},{"SortOrder":13,"Sku":"C2529","ProductName":"NiCo21(DE3) Competent E. coli"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":5,"Category":"Cell Lysis","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"P8115","ProductName":"NEBExpress® T4 Lysozyme"},{"SortOrder":2,"Sku":"P8116","ProductName":"NEBExpress® E. coli Lysis Reagent"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":6,"Category":"Purification Beads, Columns and Resins","ProductFamily":"1","ProductFamilyDescription":"Isolation of pure substrates or proteins for downstream experiments is a common, yet time consuming, task. New England Biolabs offers a variety of resins and magnetic beads that are easy-to-use, highly specific, and available in several different formats for rapid isolation and purification of proteins, nucleic acids and immunoglobulins. NEB’s magnetic beads are ideally suited for applications involving high-throughput proteomic screening, small-scale protein isolation, immunomagnetic isolations or cell separation experiments. With magnetic beads, affinity purification of tagged proteins, antigens, antibodies and nucleic acids can be done conveniently and quickly. Immobilized substrates remain biologically active and can be eluted in small volumes or serve as ligands in subsequent pull-down or target interaction experiments involving DNA or proteins. NEB’s resins enable simple, one-step purification strategies for tagged proteins, and result in a high yield of highly pure substrate. For the full list of products available for protein expression and purification, visit www.neb.com/ ProteinExpression.","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"S1423","ProductName":"NEBExpress® Ni-NTA Magnetic Beads"},{"SortOrder":2,"Sku":"S1427","ProductName":"NEBExpress® Ni Spin ColumnsNEBExpress Ni-NTA Magnetic Beads: An affinity matrix for the small-scale isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins in manual or automated formats. High specific binding yields purities of > 95% in a single-purification step. Matrix tolerates a wide range of conditions, including the presence of denaturants and detergents. Compatible with commercially available detergent-based cell lysis reagents. Elution can be achieved by protonation, ligand exchange (with imidazole) or extraction of the metal ion by a strong chelator (e.g., EDTA).
\nNEBExpress Ni Resin: NEBExpress Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure flow columns and batch purifications, and high specific binding yields purities of > 95% in a single-purification step. NEBExpress Ni Resin is comprised of a highly uniform and chemical-tolerant resin that is pre-charged with nickel ions on the matrix surface. It is resistant to a wide range of chemicals, including NaOH, EDTA, and commonly used reducing agents such as TCEP, DTT, and β-mercaptoethanol. Can be used under native or denaturing conditions.
\nNEBExpress Ni Spin Columns: NEBExpress Ni\nSpin columns are pre-packed with agarose-based\nmicroparticles ranging in size from 10-100 µm for the\nsmall-scale isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. Purification can be\nperformed under native or denaturing conditions, including conditions in which EDTA or reducing reagents are\nrequired, yielding highly pure target protein in a single\npurification step. This enables screening of expression\nconditions and streamlines the functional and structural\ncharacterization of the target protein.\n
\nTEV Protease: TEV Protease, also known as Tobacco\nEtch Virus (TEV) Protease, is a highly specific cysteine\nprotease that recognizes the amino-acid sequence\nGlu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) and cleaves\nbetween the Gln and Gly/Ser residues. It is often used\nfor the removal of affinity purification tags such as\nmaltose-binding protein (MBP) or polyhistidine from\nfusion proteins. TEV Protease has a 7XHis-tag for easy\nremoval from a reaction using nickel affinity resins and\nhas been engineered to improve thermal stability and\ndecrease autolysis.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"S1423","ProductName":"NEBExpress® Ni-NTA Magnetic Beads"},{"SortOrder":2,"Sku":"S1428","ProductName":"NEBExpress® Ni Resin"},{"SortOrder":3,"Sku":"S1427","ProductName":"NEBExpress® Ni Spin ColumnsAmylose Resin and Amylose Resin High Flow: Affinity matrix used for isolation of proteins fused to maltose-binding protein. It is a composite amylose/agarose bead. Amylose Resin High Flow is a more rigid bead, suitable for use in automated chromatography systems.
\nBinding Capacity: Amylose Resin and Amylose Resin High Flow: > 4 mg MBP5-paramyosin ∆Sal fusion protein/ml of bed volume.
\nAmylose Magnetic Beads: Affinity matrix for the small-scale isolation and purification of MBP-fusion proteins. Amylose is covalently coupled to a paramagnetic particle through a linkage that is stable and leak resistant over a wide pH range.
\nSupport Matrix: 10 µM\nsuperparamagnetic particles.\n
\nBinding Capacity: 1 mg of Amylose Magnetic Beads will\nbind ≥ 10 µg of MBP-fusion protein.
\nAnti-MBP Monoclonal Antibody: Anti-MBP\nMonoclonal Antibody is a murine anti-maltose-binding\nprotein antibody, isotype IgG2a. It is purified from tissue\nculture supernatant by protein A affinity chromatography.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"E8021","ProductName":"Amylose Resin"},{"SortOrder":2,"Sku":"E8022","ProductName":"Amylose Resin High Flow"},{"SortOrder":3,"Sku":"E8035","ProductName":"Amylose Magnetic Beads"},{"SortOrder":4,"Sku":"E8032","ProductName":"Anti-MBP Monoclonal Antibody"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":9,"Category":"Chitin Binding Domain (CBD) Purification","ProductFamily":"1","ProductFamilyDescription":"Chitin Resin: An affinity matrix for the isolation of target proteins fused on an intein-chitin binding domain (CBD). Strong specific binding enables purification of highly pure protein from crude lysates in one step. Removal of CBD-tag during elution typically yields highly pure, native protein without the use of a protease.
\nSupport Matrix: Approximately 50-70 μm microparticles
\nBinding Capacity: 2.0 mg maltose-binding protein/ ml bed volume released from the resin after cleavage of the fusion protein expressed from pMYB5.
\nChitin Magnetic Beads: An affinity matrix for the small-scale isolation of target proteins fused to a chitin binding domain (CBD). Chitin beads have been preparedwith encapsulated magnetite, thereby permitting the\nmagnetic isolation of CBD-fusion proteins from cell\nculture supernatants. Removal of CBD-tag during elution\ntypically yields highly pure, native protein.\n
\nSupport Matrix: Approximately 50-70 μm paramagnetic\nmicroparticles\n
\nBinding Capacity: 2 mg chitin binding domain protein /\nml bed volume released\n
\nAnti-CBD Monoclonal Antibody: Anti-CBD Monoclonal Antibody is a murine anti-chitin binding domain\n(CBD) antibody, isotype IgG1. It has high purity and\nspecificity for chitin binding domain tag, and is verified\nfor use in both Western blotting and ELISA.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"S6651","ProductName":"Chitin Resin"},{"SortOrder":2,"Sku":"E8036","ProductName":"Chitin Magnetic Beads"},{"SortOrder":3,"Sku":"E8034","ProductName":"Anti-CBD Monoclonal Antibody"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":10,"Category":"Magenetic Bead Purification Products","ProductFamily":"","ProductFamilyDescription":"Oligo d(T)25 Magetic Beads: These beads enable small-scale isolations of mRNA from a variety of samples, including in vitro transcribed mRNA, total RNA, crude cell lysates and tissue. The selectivity for mRNA results from the annealing of bead-linked oligo d(T)25 to the poly(A) region present in most eukaryotic mRNAs.
\nSupport Matrix: 1 μm nonporous superparamagnetic microparticles
\nBinding Capacity: ≥ 5 µg rA30 per mg of beads
\nMagnetic mRNA Isolation Kit: The Magnetic mRNA Isolation Kit is designed to isolate intact poly(A)+ RNA from cells and tissue without requiring phenol or other organic solvents. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads, which is then used as the solid support for the direct binding of poly(A)+ RNA.
\nStreptavidin Magnetic Beads: The beads provide fast magnetic response times and reaction kinetics, and they have high binding capacity and sensitivity while retaining their physical integrity. They can be used to capture biotin-labeled substrates including DNA, RNA, peptides, antigens, antibodies and other proteins of interest in manual or automated workflows. These beads typically exhibit lower non-specific binding of proteins.
\nSupport Matrix: 1 μm nonporous superparamagnetic microparticles Binding Capacity: ≥ 30 µg biotinylated antibody per mg of beads or > 500 pmol of single-stranded 25 bp biotinylated oligonucleotide per mg of beads
\nHydrophilic Streptavidin Magnetic Beads: The beads provide rapid magnetic response times and reaction kinetics, and they have high binding capacity and sensitivity while retaining their physical integrity. They can be used to capture biotin-labeled substrates including DNA, RNA, peptides, antigens, antibodies and other proteins of interest in manual or automated workflows. These beads typically exhibit lower non-specific binding of nucleic acids.
\nSupport Matrix: 2 µM non-porous superparamagnetic microparticles
\nBinding Capacity: > 400 pmol of single-stranded 25 bp biotinylated oligonucleotide per mg of beads
\nProtein A and Protein G Magnetic Beads: The beads allow for isolation of most mammalian immunoglobulins (IgGs) and are amenable to immunoprecipitation. Predominant Fc-binding allows optimal IgG orientation upon binding to the outer surface of the Protein A and Protein G Magnetic Beads allowing Fab regions to efficiently bind antigen. These beads can be used to immunoprecipitate target proteins from crude cell lysates using a selected primary antibody. In addition, specific antibodies can be chemically cross-linked to the Protein A- or Protein G- coated surface to create a reusable immunoprecipitation bead, thereby avoiding the co-elution of antibody with the target antigen.
\nSupport Matrix: 2 µm nonporous superparamagnetic microparticles
\nBinding Capacity: > 280 µg of Human IgG per ml of beads
","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"Magenetic Bead Purification Products","ProductFamily":"1","ProductFamilyDescription":"Oligo d(T)25 Magnetic Beads: These beads\nenable small-scale isolations of mRNA from a variety\nof samples, including in vitro transcribed mRNA, total\nRNA, crude cell lysates and tissue. The selectivity\nfor mRNA results from the annealing of bead-linked\noligo d(T)25 to the poly(A) region present in most\neukaryotic mRNAs.
\nMagnetic mRNA Isolation Kit: The Magnetic mRNA\nIsolation Kit is designed to isolate intact poly(A)+ RNA\nfrom cells and tissue without requiring phenol or other\norganic solvents. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads, which\nis then used as the solid support for the direct binding\nof poly(A)+ RNA.\n
\nStreptavidin Magnetic Beads: The beads provide\nfast magnetic response times and reaction kinetics, and\nthey have high binding capacity and sensitivity while\nretaining their physical integrity. They can be used to\ncapture biotin-labeled substrates including DNA, RNA,\npeptides, antigens, antibodies and other proteins of\ninterest in manual or automated workflows. These beads\ntypically exhibit lower non-specific binding of proteins.\n
\nHydrophilic Streptavidin Magnetic Beads: The\nbeads provide rapid magnetic response times and reaction kinetics, and they have high binding capacity and\nsensitivity while retaining their physical integrity. They\ncan be used to capture biotin-labeled substrates including DNA, RNA, peptides, antigens, antibodies and other\nproteins of interest in manual or automated workflows.\nThese beads typically exhibit lower non-specific binding\nof nucleic acids.\n
\nProtein A and Protein G Magnetic Beads: The\nbeads allow for isolation of most mammalian immunoglobulins (IgGs) and are amenable to immunoprecipitation. Predominant Fc-binding allows optimal\nIgG orientation upon binding to the outer surface of the\nProtein A and Protein G Magnetic Beads, enabling Fab\nregions to efficiently bind antigen. These beads can be\nused to immunoprecipitate target proteins from crude\ncell lysates using a selected primary antibody. In addition, specific antibodies can be chemically cross-linked\nto the Protein A- or Protein G- coated surface to create\na reusable immunoprecipitation bead, thereby avoiding\nthe co-elution of antibody with the target antigen.\n
\nDescription: A DH10B derivative suitable for a wide range of applications, including large plasmid and BAC cloning.
\nGenotype: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- Φ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 Δ(mrr-hsdRMS-mcrBC)\n
\nFeatures:
\nTransformation Efficiency: High Efficiency: 1–3 x 109 cfu/µg pUC19 DNA (NEB #C3019H, #C3019I); 1–3 x 108 cfu/µg pUC19 DNA (NEB #C3019P)
\nElectrocompetent: > 2 x 1010 cfu/µg pUC19 DNA
\nResistance: Resistance to phage T1 (fhuA), Str\n
\nSensitivity: Amp, Cam, Kan, Nit, Spec, Tet\n
\nReagents Supplied:
\nDescription: A DH5α derivative and versatile E. coli cloning strain.
\nGenotype: fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17
\nFeatures:
\nTransformation Efficiency:\nHigh Efficiency: 1–3 x 109\ncfu/µg pUC19 DNA\n(NEB #C2987H, #C2987I, #C2987P, #C2987U);\n1–5 x 108\ncfu/µg pUC19 DNA (NEB #C2987R)
\nSubcloning Efficiency: > 1 x 106\ncfu/µg pUC19 DNA
\nResistance: Resistance to phage T1 (fhuA2)\n
\nSensitivity: Amp, Cam, Kan, Nit, Spec, Str, Tet\n
\nReagents Supplied:
\n* NEB 5-alpha Competent E. coli (Subcloning Efficiency) is not supplied\nwith SOC Outgrowth Medium or pUC19 Control DNA.
","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"C2987","ProductName":"NEB® 5-alpha Competent E. coli (High Efficiency)"},{"SortOrder":2,"Sku":"C2988","ProductName":"NEB® 5-alpha Competent E. coli (Subcloning Efficiency)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""}],"Products":{"Products":[{"SortOrder":1,"Sku":"C2992","ProductName":"NEB® 5-alpha F'Iq Competent E. coli (High Efficiency)"},{"SortOrder":2,"Sku":"B9035","ProductName":"NEB® 10-beta/Stable Outgrowth Medium"},{"SortOrder":3,"Sku":"C1010","ProductName":"NEB® Cloning Competent E. coli Sampler
"},{"SortOrder":4,"Sku":"C2925","ProductName":"dam-/dcm- Competent E. coli"},{"SortOrder":5,"Sku":"C3040","ProductName":"NEB® Stable Competent E. coli (High Efficiency)"},{"SortOrder":6,"Sku":"B9020","ProductName":"SOC Outgrowth Medium"},{"SortOrder":7,"Sku":"C1008","ProductName":"NEB Tube Opener"},{"SortOrder":8,"Sku":"C2984","ProductName":"NEB® Turbo Competent E. coli (High Efficiency)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":2,"Category":"Protein Expression Strains","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"C2523","ProductName":"NEBExpress® Competent E. coli (High Efficiency)"},{"SortOrder":2,"Sku":"C2527","ProductName":"BL21(DE3) Competent E. coli"},{"SortOrder":3,"Sku":"C2528","ProductName":"Lemo21(DE3) Competent E. coli"},{"SortOrder":4,"Sku":"C2529","ProductName":"NiCo21(DE3) Competent E. coli"},{"SortOrder":5,"Sku":"C2530","ProductName":"BL21 Competent E. coli"},{"SortOrder":6,"Sku":"C2566","ProductName":"T7 Express Competent E. coli (High Efficiency)"},{"SortOrder":7,"Sku":"C3010","ProductName":"T7 Express lysY Competent E. coli (High Efficiency)"},{"SortOrder":8,"Sku":"C3013","ProductName":"T7 Express lysY/Iq Competent E. coli (High Efficiency)"},{"SortOrder":9,"Sku":"C3026","ProductName":"SHuffle® T7 Competent E. coli"},{"SortOrder":10,"Sku":"C3028","ProductName":"SHuffle® Express Competent E. coli"},{"SortOrder":11,"Sku":"C3029","ProductName":"SHuffle® T7 Express Competent E. coli"},{"SortOrder":12,"Sku":"C3030","ProductName":"SHuffle® T7 Express lysY Competent E. coli"},{"SortOrder":13,"Sku":"C3037","ProductName":"NEBExpress® Iq Competent E. coli (High Efficiency)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""}],"Products":{"Products":[]},"ImageMetadata":[],"Charts":[{"ChartKind":"Static","ChartTitle":"Competent Cell Selection Guide","ChartCode":"Competent Cell Selection Guide"}],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":11,"Category":"Glycobiology & Protein Analysis Tools
","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"Endoglycosidases","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"Endo H","ProductFamily":"1","ProductFamilyDescription":"Description: Endoglycosidase H is a recombinant glycosidase which cleaves within the chitobiose core of high mannose and some hybrid oligosaccharides from N-linked glycoproteins.\n
\nEndo Hf is a recombinant protein fusion of Endoglycosidase H and maltose binding protein. It has identical activity to Endo H.\n
\nSource: Endo H and Endo Hf have been cloned from Streptomyces plicatus and overexpressed in E. coli.\n
\nReaction Conditions: Denature glycoprotein in 1X Glycoprotein Denaturing Buffer at 100°C for 10 minutes. Incubate in 1X GlycoBuffer 3 at 37°C. Heat inactivation: 65°C for 10 minutes.\n
\nReagents Supplied:
\nMolecular Weight:
\nUnit Definition: One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 µl.\n
\nConcentration:\nEndo H concentration: 500,000 units/ml, Endo Hf concentration: 1,000,000 units/ml\n
\nNote: Enzymatic activity is not affected by SDS. To deglycosylate a native glycoprotein, longer incubation time as well as more enzyme may be required.
","ProductFamilyIconCharacters":["7","n","r","w",">"],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"P0702","ProductName":"Endo H"},{"SortOrder":2,"Sku":"P0703","ProductName":"Endo Hf"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":2,"Category":"PNGase F & PNGase F, Recombinant","ProductFamily":"1","ProductFamilyDescription":"Description: Peptide-N-Glycosidase F, also known as PNGase F, is an amidase which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins. A glycerol-free version of PNGase F is also offered for HPLC methods.
\nSource: NEB #P0704 and #P0705 are purified from Elizabethkingia miricola (formerly Flavobacterium meningosepticum).
\nNEB #P0708 and #P0709 are purified from Elizabethkingia miricola (formerly Flavobacterium meningosepticum) and expressed in E. coli.
\nReaction Conditions: Denature glycoprotein in 1X Glycoprotein Denaturing Buffer at 100°C for 10 minutes. Heat inactivation: 75°C for 10 minutes.
\nReagents Supplied:
\nMolecular Weight: 36 kDa.
\nUnit Definition: One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 µl.
\nConcentration: 500,000 units/ml
\nNote: Since PNGase F activity is inhibited by SDS, it is essential to have NP-40 present in the reaction mixture.
\nTo deglycosylate a native glycoprotein, longer incubation time as well as more enzyme may be required.
","ProductFamilyIconCharacters":["7","n","r","w","?"],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"P0704","ProductName":"PNGase F"},{"SortOrder":2,"Sku":"P0705","ProductName":"PNGase F (Glycerol-free)"},{"SortOrder":3,"Sku":"P0708","ProductName":"PNGase F, Recombinant"},{"SortOrder":4,"Sku":"P0709","ProductName":"PNGase F (Glycerol-free), Recombinant"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":3,"Category":"Rapid™ PNGase F & Rapid PNGase F (non-reducing format)","ProductFamily":"1","ProductFamilyDescription":"Description: Rapid PNGase F is an improved reagent that allows the complete and rapid deglycosylation of antibodies and fusion proteins in minutes. All N-glycans are released rapidly and without bias, and are ready to be prepared for downstream chromatography or mass spectrometry analysis. Rapid PNGase F creates an optimized workflow which reduces processing time without compromising sensitivity or reproducibility.\n
\nDeveloped for proteomic applications, Rapid PNGase F (non-reducing format) enables complete and rapid deglycosylation while preserving disulfide bonds. This facilitates high-throughput proteomics applications and methods for antibody characterization by mass spectrometry such as intact mass analysis. Rapid PNGase F (non-reducing format) combines the advantages of Rapid PNGase F (fast processing time), with non-reducing conditions, preserving quaternary structure.\n
\nHeat inactivation: 75°C for 10 minutes.\n
\nReagents Supplied (NEB #P0710):
\nReagents Supplied (NEB #P0711):
\nSpecificity: Rapid PNGase F cleaves all complex, hybrid and high-mannose type glycans from antibodies and related proteins. Core α1-3 fucosylation (found in immunoglobulins expressed in plant or insect cells) is resistant to both PNGase F and Rapid PNGase F.
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As the number of protein kinases grows, identifying their substrates has become more challenging. Consensus phosphorylation motifs, derived from amino acid sequences of known substrates, aid in predicting kinase-substrate interactions. However, these motifs oversimplify kinase specificity, ignoring complex 3D interactions and secondary/tertiary structural elements. Not all residues in these motifs contribute equally to kinase recognition, so caution is needed. Despite this, consensus motifs are valuable for predicting phosphorylation sites and serve as useful substrates for kinase assays. The table below summarizes protein kinase specificity motifs for protein kinases available from NEB, with interchangeable amino acids separated by slashes (/) and weakly recognized residues marked with “X.”","ProductFamilyIconCharacters":[],"SubCategories":[],"Products":{"Products":[{"SortOrder":1,"Sku":"P6000","ProductName":"cAMP-dependent Protein Kinase (PKA), catalytic subunit"},{"SortOrder":2,"Sku":"P6010","ProductName":"Casein Kinase II (CK2)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"1","DisplayAsTableColumns":[{"FieldName":"FullProductName","ColumnDisplayName":"Product"},{"FieldName":"CatalogNumber","ColumnDisplayName":"NEB #"},{"FieldName":"Size","ColumnDisplayName":"Size"}],"GroupFieldName":""}],"Products":{"Products":[{"SortOrder":1,"Sku":"P0753","ProductName":"Lambda Protein Phosphatase (Lambda PP)"},{"SortOrder":2,"Sku":"P6000","ProductName":"cAMP-dependent Protein Kinase (PKA), catalytic subunit"},{"SortOrder":3,"Sku":"P6010","ProductName":"Casein Kinase II (CK2)"}]},"ImageMetadata":[],"Charts":[],"DisplayAsTable":"","DisplayAsTableColumns":[],"GroupFieldName":""},{"SortOrder":6,"Category":"Phage Display","ProductFamily":"","ProductFamilyDescription":"","ProductFamilyIconCharacters":[],"SubCategories":[{"SortOrder":1,"Category":"Ph.D.™ Phage Display Peptide Library Kits","ProductFamily":"1","ProductFamilyDescription":"Description: Phage display describes a selection technique in which a library of peptide or protein variants is expressed on the outside of a phage virion, while the genetic material encoding each variant resides on the inside. This creates a physical linkage between each variant protein sequence and the DNA encoding it, which allows rapid partitioning based on binding affinity to a given target molecule (antibodies, enzymes, cell-surface receptors, etc.) by an in vitro selection process called panning. In its simplest form (Figure 1), panning is carried out by incubating a library of phage-displayed peptides with a plate (or bead) coated with the target, washing away the unbound phage, and eluting the specifically-bound phage. The eluted phage is then amplified and taken through additional binding/amplification cycles to enrich the pool in favor of binding sequences. After 3–4 rounds, individual clones are characterized by DNA sequencing and ELISA. The Ph.D. v2 kits have been updated with a new control panning target for an optional epitope mapping experiment.
\nNEB offers 3 pre-made random peptide libraries, as well as the cloning vector M13KE for construction of custom libraries. The pre-made libraries consist of linear heptapeptide (Ph.D.-7) and dodecapeptide (Ph.D.-12) libraries, as well as a disulfide-constrained heptapeptide (Ph.D.-C7C) library. All of the libraries have complexities in excess of 2 billion independent clones. The randomized peptide sequences in all three libraries are expressed at the N-terminus of the minor coat protein pIII, resulting in a valency of 5 copies of the displayed peptide per virion.\n
\nThe Ph.D. libraries have been used for myriad applications, including epitope mapping (Figure 2), identification of protein-protein contacts and enzyme inhibitors and discovery of peptide ligands for GroEL, HIV, semiconductor surfaces and small-molecule fluorophores and drugs.
\nThe Ph.D. Kits Include:
\nDescription: While bisulfite sequencing has been the gold standard for the study of DNA methylation, this conversion treatment is damaging to DNA, resulting in DNA fragmentation, loss and GC bias. The NEBNext Enzymatic Methyl-seq Kit (EM-seq) provides an enzymatic alternative to whole genome bisulfite sequencing (WGBS), combined with high-efficiency streamlined library preparation suitable for Illumina sequencing.\n
\nThe highly effective EM-seq enzymatic conversion minimizes damage to DNA and, in combination with the supplied NEBNext Ultra II library preparation workflow reagents, results in high quality libraries that enable superior detection of 5mC and 5hmC from fewer sequencing reads. Products specific for 5hmC detection (E5hmc-seq™) are also available.
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\nThe new NEBNext Enzymatic Methyl-seq v2 Kit has a wider input range (as low as 100 ng) and a faster, more streamlined workflow than the original EM-seq kit (NEB #E7120).
\nThe NEBNext Enzymatic Methyl-seq v2 Kit includes conversion reagents, library prep reagents and the EM-seq Adaptor. Multiole sets of the required index primers (NEBNext LV Unique Dual Index Primers) are available separately, enabling greater flexibility in multiplexing.
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\nSNAP-Cell Block is highly membrane permeant and once inside the cell reacts with the SNAP-tag, irreversibly inactivating it for subsequent labeling steps.
\nSNAP-Surface Block also reacts with the SNAP-tag irreversibly, inactivating it for subsequent labeling steps. This blocker is largely membrane impermeant essentially limiting blocking to cell surface-exposed SNAP-tags.
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\nThe bacterial expression vector pSNAP-tag(T7)-2 includes cloning sites both upstream and downstream from the SNAP-tag, which is under the control of the T7 promoter. Codon usage in the SNAP-tag gene has been optimized for E. coli expression.
\nSource: Isolated from an E. coli strain by a standard plasmid purification procedure.
\nConcentration: 500 µg/ml
\nRestriction Map: The restriction map for pSNAPf Vector can be found in the Technical Reference section. Additional sequence and map files for expression and control plasmids can be found at www.neb.com.
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\nBIACORE® is a registered trademark of GE Healthcare Life Sciences
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